中山醫學大學機構典藏 CSMUIR:Item 310902500/3811
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    題名: Studies of the effect of ionomycin on the KCNQ4 channel expressed in Xenopus oocytes
    作者: Ching-Chyuan Su;Shuan-Yow Li;Jiann-Jou Yang;Mao-Chang Su;Min-Jon Lin
    貢獻者: 中山醫學大學:醫學系
    關鍵詞: KCNQ4;Ionomycin;Calcium;BAPTA-AM;Channel
    日期: 2006
    上傳時間: 2011-06-13T07:30:37Z (UTC)
    摘要: The effect of ionomycin on the human KCNQ4 channels expressed in Xenopus leavis oocytes was investigated. KCNQ4 channels expressed in Xenopus oocytes were measured using two-electrode voltage clamp. The activation of KCNQ4 current had slow activation kinetics and low threshold (not, vert, similar?50 mV). The expressed current of KCNQ4 showed the half-maximal activation (V1/2) was ?17.8 mV and blocked almost completely by KCNQ4 channel blockers, linopirdine (300 μM) or bepridil (200 μM). The significant increase of KCNQ4 outward current induced by ionomycin (calcium salt) is about 1.7-fold of control current amplitude at +60 mV and shifted V1/2 by approximately ?8 mV (from ?17.8 to ?26.0 mV). This effect of ionomycin could be reversed by the further addition of BAPTA-AM (0.3 mM), a membrane–permeable calcium chelator. Furthermore, the increased effect of ionomycin on KCNQ4 current is abolished by pretreatment of linopirdine or bepridil. In contrast, direct cytoplasmic injection of calcium medium (up to 1 mM calcium, 50 nl) did not mimic the effect of ionomycin. In conclusion, the effect of ionomycin on enhancement of KCNQ4 current is independent of intracellular calcium mobilization and possibly acts on intramembrane hydrophobic site of KCNQ4 protein expressed in Xenopus oocytes.
    URI: https://ir.csmu.edu.tw:8080/ir/handle/310902500/3811
    http://dx.doi.org/10.1016/j.bbrc.2006.07.053
    關聯: Biochemical and Biophysical Research Communications,Volume 348, Issue 1, 15 September 2006, Pages 295-300
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