Molecular imaging of in vivo calcium ion expression in area postrema of total sleep deprived rats: Implications for cardiovascular regulation by TOF-SIMS analysis

doi:10.1016/j.apsusc.2010.01.066

中山醫學大學機構典藏 CSMUIR > 醫學院 > 醫學系 > 期刊論文 > Item 310902500/3793

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题名:	Molecular imaging of in vivo calcium ion expression in area postrema of total sleep deprived rats: Implications for cardiovascular regulation by TOF-SIMS analysis
作者:	Fu-Der Mai;Li-You Chen;Yong-Chien Ling;Bo-Jung Chen;Un-In Wu;Hung-Ming Chang
贡献者:	中山醫學大學:醫學系
关键词:	Calcium;Sleep deprivation;Area postrema;TOF-SIMS;Cardiovascular regulation;Quantitative immunohistochemistry
日期:	2010
上传时间:	2011-05-20T08:45:06Z (UTC)
ISSN:	0169-4332
摘要:	Excessive calcium influx in chemosensitive neurons of area postrema (AP) is detrimental for sympathetic activation and participates in the disruption of cardiovascular activities. Since total sleep deprivation (TSD) is a stressful condition known to harm the cardiovascular function, the present study is aimed to determine whether the in vivo calcium expression in AP would significantly alter following TSD by the use of time-of-flight secondary ion mass spectrometry (TOF-SIMS) and calretinin (a specific calcium sensor protein in AP neurons) immunohistochemistry. The results indicated that in normal rats, the calcium intensity was estimated to be 0.5 × 105 at m/z 40.08. However, following TSD, the intensity for calcium ions was greatly increased to 1.2 × 105. Molecular imaging revealed that after TSD, various strongly expressed calcium signals were distributed throughout AP with clear identified profiles instead of randomly scattered within this region in normal rats. Immunohistochemical staining corresponded well with ionic image in which a majority of calcium-enriched gathering co-localized with calretinin positive neurons. The functional significance of TSD-induced calcium augmentation was demonstrated by increased heart rate and mean arterial pressure, clinical markers for cardiovascular dysfunction. Considering AP-mediated sympathetic activation is important for cardiovascular regulation, exaggerated calcium influx in AP would render this neurocircuitry more vulnerable to over-excitation, which might serve as the underlying mechanism for the development of TSD-relevant cardiovascular deficiency.
URI:	https://ir.csmu.edu.tw:8080/handle/310902500/3793 http://dx.doi.org/10.1016/j.apsusc.2010.01.066
關聯:	Applied Surface Science,Volume 256, Issue 14, 1 May 2010, Pages 4456-4461
显示于类别:	[醫學系] 期刊論文

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