Expression and localization of Tmie in adult rat cochlea

doi:10.1007/s00418-008-0385-z

中山醫學大學機構典藏 CSMUIR > 醫學科技學院 > 生物醫學科學學系暨碩士班 > 期刊論文 > Item 310902500/3642

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.csmu.edu.tw:8080/ir/handle/310902500/3642

题名:	Expression and localization of Tmie in adult rat cochlea
作者:	Su MC;Yang JJ;Chou MY;Hsin CH;Su CC;Li SY
贡献者:	中山醫學大學:生物醫學科學學系(所)
关键词:	Tmie;Immunohistochemistry;Laser capture microdissection (LCM);Cochlea;Deafness
日期:	2008
上传时间:	2011-03-22T08:13:52Z (UTC)
ISSN:	0948-6143
摘要:	Loss-of function mutations in transmembrane inner ear expressed (Tmie/TMIE) gene have been shown to cause deafness in mice and humans (DFNB6). However; the functional roles of TMIE in the cochlea remain unclear. A primary step toward the understanding of the role of TMIE in hearing and its dysfunction is the documentation of its cellular and sub-cellular location within the cochlea; the auditory organ. In this study; we located and determined the cellular expression of Tmie within the rat cochlea using a polyclonal anti-Tmie antibody. The anti-Tmie antibody identified a specific band of 17 kDa in a variety of rat tissues by using Western blot analyses. The expression products of Tmie were also detected in the spiral limbus; spiral ligament; organ of Corti; and stria vascularis by immunohistochemistry analysis and RT-PCR. Our results point out the presence and localization of Tmie products in the cochlea of rat. Knowledge of spatial distribution of Tmie will provide important insight into the mechanisms that lead to deafness due to mutations in the TMIE gene.
URI:	https://ir.csmu.edu.tw:8080/handle/310902500/3642 http://dx.doi.org/10.1007/s00418-008-0385-z
關聯:	Histochemistry and Cell Biology. 130; 119-126.
显示于类别:	[生物醫學科學學系暨碩士班] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html	期刊論文	0Kb	HTML	557	检视/开启
index.html		0Kb	HTML	398	检视/开启

在CSMUIR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....