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https://ir.csmu.edu.tw:8080/ir/handle/310902500/3370
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| 题名: | 台灣地區腦腫瘤形成與人類多瘤性病毒感染相關性之研究
Relationship between CNS Neoplasm Pathogenesis and Human Polymavirus, JCV, in Taiwan |
| 作者: | 張菡
Chang, Han |
| 贡献者: | 中山醫學院附設醫院病理科 |
| 关键词: | JC病毒;人類多瘤性病毒;腦部腫瘤
JC virus;Human polyomavirus;Brain tumor |
| 日期: | 2001 |
| 上传时间: | 2010-12-17T03:14:45Z (UTC)
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| 摘要: | 人類多瘤性病毒（Human polyomavirus），JC病毒，可感染人類腦細胞。其感染的機制可分為生產型（Permissive）及非生產型（Non-permissive）。其中生產型的感染機制是當JC病毒感染腦細胞及其腫瘤抗原（Large tumor antigen，Tag）表現後，出現病毒之去氧核糖核酸複製，病毒顆粒之形成，最後導致宿主細胞的溶解與破壞；若感染腦神經棘細胞（Oligodendrocyte）並產生溶解而導致進行性多病兆的腦白質病（Progressive multifocal leukoencephalopathy；PML）。然而，病毒腫瘤抗原之表現後，並沒有出現病毒去氧核糖核酸之複製，卻與宿主調節蛋白質發生作用，引起細胞癌化（Malignant transformation）及無法控制的細胞增生，則被稱為非生產型感染機制。JC病毒去氧核糖核酸和腫瘤抗原核糖核酸曾被發現於腦腫瘤中。我們企圖瞭解在台灣地區人類多瘤性病毒，JC病毒，與腦腫瘤發生之相關性。我們使用48例腦腫瘤以Solution polymerase chain reaction及去氧核糖核酸定序的方法偵測JC病毒。我們利用免疫組織化學法偵測腫瘤抗原和結構蛋白。從實驗結果中，我們證實確實有JCV DNA的存在於8％（448）腦腫瘤組織中。更重要地，JCV Tag表現於33％（16/48）腦腫瘤中。在所有測試檢體中，沒有結構蛋白的存在。這些發現支持JCV病毒具有細胞癌化的潛能。
The characteristic of human polyomavirus, JCV, is able to infect human brain glial cells. The response to infection can be permissive or nonpermissive. It has been reported that JCV can induce a productive infection of oligodendrocytes resulting in human progressive multifocal leukoencephalopathy (PML). The mechanism has been suggested that JCV T-antigen expression orchestrate events leading to viral replication, virion formation, and subsequent lysis and destruction of the host cells. However, if JCV T-antigen is expressed in a cell in the absence of viral replication, large tumor antigen (Tag) may interact with host regulatory proteins to result in cellular transformation and ultimately uncontrolled proliferation. Recent reported that JCV DNA and Tag RNA have been demonstrated in human CNS neoplasms of different histopathologic types. To investigate the role of human polyomavirus infection in CNS neoplasm pathogenesis in Taiwan, we used a PCR method with a pair of primers that recognize the regulatory region of human polyomavirus DNA sequence in clinical specimens of 48 brain tumors including astrocytomas (grade I to IV), medulloblastoma/primitive neuroectodermal tumors, schwannomas, meningiomas, malignant lymphomas and metastatic carcinomas, as well as 9 cerebral benign lesions such as gliosis, abscess and arterio-venous malformation. Only 4 cases were PCR-positive, 2 in astrocytomas and 2 in metastatic carcinomas. Importantly, immunohistochemistry was further performed with primary antibodies of anti-Tag and anti-JCV VP1. The result revealed the Tag expression of tumor cells in 56% astrocytomas, 22% medulloblastoma/PNETs, 40% schwannomas and 27% metastatic carcinomas. One of 9 benign brain lesions contained the Tag. There was no evidence of VP1 expression in brain tumors and benign lesions. The results support that JCV infects and potentially transforms neuroglial cells. |
| URI: | https://ir.csmu.edu.tw:8080/handle/310902500/3370 |
| 显示于类别: | [病理科] 研究計劃
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