臨床上經常用來診斷腎臟病病人的四種非蛋白氮化合物:尿素、尿酸、肌酸與肌酸肝可以被毛細管電泳予以鑑定分離。此四種非蛋白氮化合物在磷酸鹽溶液pH7.4,電壓30KV的條件下在30分鐘內成功的被分離。
The capillary electrophoretic separation of the four nonprotein nitrogenous compounds (urea, uric acid, creatine and creatinine) typically employed in clinical/medical settings for monitoring renal function. Successful resolution of these compounds was achieved via use of a bile salt micelle system composed of sodium cholate at phosphate buffer (pH 7.4). The elution patterns of four NPNs can be obtained within thirty minutes with a voltage of 30 KV. The effect of varying the applied voltage, temperature and the mole ratio of phosphate buffer with bile salt surfactant on the migration behavior is also examined.
