v-Ras癌化的細胞，其細胞架構較諸正常細胞產生了巨大的變化，且整體蛋白的Tyrosyl phosphorylation較正常細胞有明顯增加。本實驗室進一步研究結果，發現在v-Ras癌化的細胞，其c-Src的kinase activity是增加的。而Wortmannin處理下的v-Ras癌化的細胞，其c-Src kinase activity是下降的。有趣的是c-Src的substrate，p97/sup Eps8/，是介於Ras和Rac之間的mediator。而我們發現p97/sup Eps8/ overexpression會造成Cell transformation。
Studies of v-Ras transformed cells revealed their altered cytoskeletal network and enhanced tyrosyl phosphorylation of total cellular proteins relative to normal cells. Further studies indicated that the activity of c-Src was increased in cells expressing v-Ras and decreased c-Src activity was observed in Wortmannin-treated v-Ras transformed cells. Interestingly, the substrate of Src, p97/sup Eps8/, was an important mediator between Ras and Rac. We observed that overexpression of p97/sup Eps8/ can lead to cellular transformation.
