建立保健食品降低血液異常發生率之評估方法

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 研究計劃 > Item 310902500/3115

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/3115

Title:	建立保健食品降低血液異常發生率之評估方法
Authors:	王朝鐘;張雲菁;黃惠珮;歐靜慈 Wang, Chau-Jong;Chang, Yun-Ching;Huang, Hui-Pei;Ou, Ting-Tsz
Contributors:	中山醫學大學生物化學研究所
Keywords:	血液異常;血癌;評估方法;洛神花;保健食品 Blood disorders;Leukemia;Evaluation method;Hibiscus sabdariffa Linne (Malvaceae);Healthy food
Date:	2010
Issue Date:	2010-12-07T09:24:17Z (UTC)
Abstract:	血液異常之發生率有逐年增加的趨勢，尤其是血癌，本年度我們進行血癌模式作為建立保健食品之評估方法，利用N-Nitroso-N-Methylurea（NMU）致雄性大白鼠（Sprague-Dawley rat）血癌之模式，進行洛神花之花青素（Hibiscus anthocyanins，HAs）抑制老鼠血癌之作用。根據血液分析、生化分析、H&E染色、骨髓性過氧化染色等實驗方法，結果發現，HAs可以降低白血球侵入肝臟及脾臟，且進一步抑制大鼠罹患血癌。由以上的結果發現HAs可降低血癌（33.3%）的作用。再者，利用HL-60細胞實驗（in vitro）探討作用機轉，結果發現HAs促進HL-60細胞凋謝死亡（apoptosis），而且，亦觀察到細胞內cytochrome c, tBid, Fas, FasL表現量增加，caspase activity亦上升，而導致細胞走向凋亡的路徑。由此我們推測HAs抑制HL-60細胞生長的作用機轉，可能是透過Fas/FasL訊息傳遞路徑，且進一步導致細胞凋亡。最後，利用inoculation方式，以尾靜脈注射WEHI-3於BALB/c小鼠體內，28天後成功地誘導小鼠產生血癌，探討抑癌之作用。同樣根據血液分析、H&E染色等分析，由結果得知，HAs可以降低白血球侵入肝臟及脾臟，且進一步抑制小鼠罹患血癌，且HAs可降低血癌的作用。希望以血癌細胞及動物實驗模式結果，建立保健食品作為預防或延緩罹患血癌之評估方法，並以洛神花萃取物證實其在延緩血癌行程評估模式之可行性。 The incidence of blood disorders, there is a rising trend, particularly leukemia. This year work has established leukemia model as health food of the assessment method. We employed the model N-Nitroso-N-Methylurea (NMU)-induced leukemia of Sprague-Dawley rat to explore inhibition of Hibiscus anthocyanins (HAs). Based on the data of blood analysis, biochemical analysis, H&E stain and MPO stain, we further demonstrate that HAs inhibits rat leukemia induced by N-nitrosomethylurea and reduces invasion of liver and spleen. We suggested that HAs could reduce leukemia progression (33.3%). Furthermore, we used human leukemia cancer cells HL-60 to explore the mechanism of HAs (in vitro). The result showed that HAs can caused HL-60 cells apoptosis, and increased cytochrome c, tBid, Fas and FasL expression and stimulation of caspase activity in HAs treated HL-60 cells. Therefore, we suggested that HAs-mediated HL-60 apoptosis via Fas-FasL pathway. Finally, we used WEHI-3/BALB/c leukemia mice model, the animals were induced by tail vein injecting marine WEHI-3 cells and allowed 28 days to develop leukemia. The same as NMU-induced leukemia model, based on the data of blood analysis and H&E stain, we further demonstrate that HAs inhibits rat leukemia induced by WEHI-3 cells and reduces invasion of liver and spleen. We suggested that HAs could reduce leukemia progression. Herein, we want to setup a series of evaluating methods for health caring foods on preventing or decelerating leukemia. Furthermore, we will also use Hibiscus sabdariffa extract to prove the possibility on delaying leukemia.
URI:	https://ir.csmu.edu.tw:8080/handle/310902500/3115
Appears in Collections:	[生化微生物免疫研究所] 研究計劃

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