人類肝細胞癌內蛋白激酵素C 異構體mRNA 之表現 The expressions of protein kinase C isoform mRNAs in human hepatocellular carcinoma

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Title:	人類肝細胞癌內蛋白激酵素C 異構體mRNA 之表現 The expressions of protein kinase C isoform mRNAs in human hepatocellular carcinoma
Authors:	謝逸憲
Contributors:	中山醫學大學:生物化學所;劉哲育
Date:	2001-08
Issue Date:	2024-05-02T01:51:32Z (UTC)
Abstract:	肝癌(Hepatocellular carcinoma ' HCC) 在亞洲是一種非常普遍的癌症，而且在台灣地藍的死亡率則是高居榜首。本實驗在最近的研究顯示肝癌的細胞棋中PKC 的活性比正當肝細胞的細胞騏中PKC 的活性低，且隨著肝癌組織的惡化其活性會隨之降低，此種現象與腫瘤大小及癌化的程度有關。本實驗想要確定PKC 異構物在肝癌中扮演的角色。因此利用RT-PCR 的技術來探討肝癌組織中PKC 異構物和細胞增殖指標mRNA 的表現。首先萃取的組肝癌組織及肝細胞癌鄰近正常組織的mRNA' 然後採用RT-PCR 技術來偵測PKC 異構物和細胞增殖指標在肝癌組織的表現章，同時以DNA 定序儀定序確定其專一性。結果發現肝癌組織內PKCα mRNA 表現量比肝癌鄰近正常組織的表現量有明顯的增加，表示肝癌組織內PKCα 正處在活化的狀態。此外肝癌組織內CPKCò 、PKCE 、PKCμ 及PKCÇmRNA 表現量增加。另外PKC 下游基因( C-raf-l 、MEK 、MAPK) 的蛋白表現量和細胞增鐘指標(Cdc2 、PCNA 及cyclin Dl ) m甜吋A 的表現量增加，因此證明PKC 異構物mRNA 的過度表現( overexpression )可能參與在肝癌形成的過程。 Human hepatocellular carcinoma (HCC) is one of the most common cancers in Asia. Our recent data had also showed that the decreased levels of membrane-bound PKC activity and PKCαprotein in HCC were correlated with tumor stage and tumor size . To verif扯the role of PKC in HCC , this study was further determined the mRNA level of PKC isoforms and cell proliferation markers in HCC by use RT-PCR. The total mRNA was isolated from the surgical specimens of 43 patients of HCC and adjacent normal tissues. The product ofRT-PCR was also checked by DNA sequence analysis . The result showed that the level PKCαmRNA in the cancer tissue were significantly higher than that in the a其jacent normal tissue , specifying that the alteraions in the PKCαmay slgm命 their activation in liver cancer . Inaddition , the level of PKCù 、PKCε 、 PKCIl and PKCÇ mRNA in the cancer tissue were also significantly higher than that in the adjacent normal tissue . Moreover , the protein expressions of the PKC down-stream gene ( c棚raf 、MEK 、MAPK) and the mRNA expression of the cell proliferation markers were also increased in cancer tissues. Thus , we suggested that the over-expression of PKC isoforms may be involved in the malignant pro皂ression of HCC.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/25808
Appears in Collections:	[The Institute of Biochemistry, Microbiology and Immunology ] Electronic Theses of Dissertation

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