肝癌(Hepatocellular carcinoma ' HCC) 在亞洲是一種非常普遍的癌
症，而且在台灣地藍的死亡率則是高居榜首。本實驗在最近的研究顯
示肝癌的細胞棋中PKC 的活性比正當肝細胞的細胞騏中PKC 的活性
低，且隨著肝癌組織的惡化其活性會隨之降低，此種現象與腫瘤大小
及癌化的程度有關。本實驗想要確定PKC 異構物在肝癌中扮演的角
色。因此利用RT-PCR 的技術來探討肝癌組織中PKC 異構物和細胞
增殖指標mRNA 的表現。首先萃取的組肝癌組織及肝細胞癌鄰近正
常組織的mRNA' 然後採用RT-PCR 技術來偵測PKC 異構物和細胞
增殖指標在肝癌組織的表現章，同時以DNA 定序儀定序確定其專一
性。結果發現肝癌組織內PKCα mRNA 表現量比肝癌鄰近正常組織
的表現量有明顯的增加，表示肝癌組織內PKCα 正處在活化的狀態。
此外肝癌組織內CPKCò 、PKCE 、PKCμ 及PKCÇmRNA 表現量增加。
另外PKC 下游基因( C-raf-l 、MEK 、MAPK) 的蛋白表現量和細胞
增鐘指標(Cdc2 、PCNA 及cyclin Dl ) m甜吋A 的表現量增加，因此
證明PKC 異構物mRNA 的過度表現( overexpression )可能參與在肝癌形成的過程。
Human hepatocellular carcinoma (HCC) is one of the most common
cancers in Asia. Our recent data had also showed that the decreased levels
of membrane-bound PKC activity and PKCαprotein in HCC were
correlated with tumor stage and tumor size . To verif扯the role of PKC in
HCC , this study was further determined the mRNA level of PKC
isoforms and cell proliferation markers in HCC by use RT-PCR. The total
mRNA was isolated from the surgical specimens of 43 patients of HCC
and adjacent normal tissues. The product ofRT-PCR was also checked by
DNA sequence analysis . The result showed that the level PKCαmRNA
in the cancer tissue were significantly higher than that in the a其jacent
normal tissue , specifying that the alteraions in the PKCαmay slgm命
their activation in liver cancer . Inaddition , the level of PKCù 、PKCε 、
PKCIl and PKCÇ mRNA in the cancer tissue were also significantly
higher than that in the adjacent normal tissue . Moreover , the protein
expressions of the PKC down-stream gene ( c棚raf 、MEK 、MAPK) and the
mRNA expression of the cell proliferation markers were also increased in
cancer tissues. Thus , we suggested that the over-expression of PKC
isoforms may be involved in the malignant pro皂ression of HCC.
