本研究利用電沉積的方式，於鈦金屬基材表面沉積奈米金微粒，並於其上固定精胺酸─甘胺酸─天門冬胺酸─半胱胺酸胜序列。使用薄膜X光繞射儀、場發掃描式電子顯微鏡、高解析度X光光電能譜儀、電化學阻抗分析法及循環伏安法對鍍有奈米金之鈦基材表面及胜結合效果進行性質分析。實驗結果顯示使用電沉積的方式，可在鈦基材表面沉積上奈米金顆粒，於不同電位下進行電沉積後，便可得到粒徑大小分別約為60 nm、110 nm及80 nm之奈米金顆粒。其相組成屬於多晶型，結晶面包括（111）、（200）、（220）及（311）。X光光電能譜儀結果則顯示胜可藉由自組裝的方式被固定於鍍有奈米金之鈦基材上。證明此種表面修飾法可作為胜或生物分子固定之替代方法。MG63類骨母細胞貼附實驗顯示所披覆上之RGDC，對於細胞貼附狀況沒有明顯的效果。
The objective of the study was to evaluate the feasibility of nano-structured Au deposited onto titanium substrates using electrodeposition. After which, immobilization of Alginine–Glycine–Aspartate–Cysteine (RGDC) peptide onto nanogold-coated titanium surface was further performed for implant applications. Characterization of the Au nano-particles electrodeposited Ti and peptide immobilization were performed using a thin film X-ray diffractometer (TFXRD), field emission scanning electron microscope (SEM), and high resolution X-ray photoelectron spectroscopy (XPS), in addition to electrochemical methods including electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The results showed that Au nano-particles could be easily electrodeposited on titanium surface. The mean particles size of 60 nm, 110 nm and 80 nm would be obtained following electrodeposition at different applied potential, respectively. XRD patterns indicated a polycrystalline orientation of the gold phase. The characteristic peaks were ascribed to (111), (200), (220), and (311) crystal faces. XPS spectrum illustrated that the RGDC peptide could be successfully immobilized on nano-gold-deposited Ti substrates via self-assembly of Au-S bonding. This procedure might be used as an alternative way for peptide or other bioactive molecules immobilization. However, the results of MG63 osteoblast-like cells cultured onto RGDC-Au-coated titanium surface showed that it did not benefit for cells attachment.
