Regenerative Potential of Platelet-Rich Fibrin Releasate Combined with Adipose Tissue-Derived Stem Cells in a Rat Sciatic Nerve Injury Model

doi:10.1177/0963689720919438

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/24926

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/24926

Title:	Regenerative Potential of Platelet-Rich Fibrin Releasate Combined with Adipose Tissue-Derived Stem Cells in a Rat Sciatic Nerve Injury Model
Authors:	Chuang, MH;Ho, LH;Kuo, TF;Sheu, SY;Liu, YH;Lin, PC;Tsai, YC;Yang, CH;Chu, CM;Lin, SZ
Keywords:	adipose tissue-derived stem cells;platelet-rich fibrin releasate;sciatic nerve injury;transplantation
Date:	2020
Issue Date:	2022-08-09T08:10:16Z (UTC)
Publisher:	SAGE PUBLICATIONS INC
ISSN:	0963-6897
Abstract:	Sciatic nerve injuries, not uncommon in trauma with a limited degree of functional recovery, are considered a persistent clinical, social, and economic problem worldwide. Accumulating evidence suggests that stem cells can promote the tissue regeneration through various mechanisms. The aim of the present study was to investigate the role of adipose tissue-derived stem cells (ADSCs) and combine with platelet-rich fibrin releasate (PRFr) in the regeneration of sciatic nerve injury in rats. Twenty-four Sprague-Dawley rats were randomly assigned to four groups, a blade was used to transect the left hindlimb sciatic nerve, and silicon tubes containing one of the following (by injection) were used to bridge the nerve proximal and distal ends (10-mm gap): group 1: untreated controls; group 2: PRFr alone; group 3: ADSCs alone; group 4: PRFr + ADSCs-treated. Walking function was assessed in horizontal rung ladder apparatus to compare the demands of the tasks and test sensitivity at 1-mo interval for a total of 3 mo. The gross inspection and histological examination was performed at 3 mo post transplantation. Overall, PRFr + ADSCs-treated performed better compared with PRFr or ADSCs injections alone. Significant group differences of neurological function were observed in ladder rung walking tests in all treated groups compared to that of untreated controls (P < 0.05). This injection approach may provide a successfully employed technique to target sciatic nerve defects in vivo, and the combined strategy of ADSCs with PRFr appears to have a superior effect on nerve repair.
URI:	http://dx.doi.org/10.1177/0963689720919438 https://www.webofscience.com/wos/woscc/full-record/WOS:000573455300045 https://ir.csmu.edu.tw:8080/handle/310902500/24926
Relation:	CELL TRANSPLANTATION ,2020 ,v29
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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