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https://ir.csmu.edu.tw:8080/ir/handle/310902500/24766
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| Title: | Anti-Apoptotic Effects of Diosgenin in D-Galactose-Induced Aging Brain |
| Authors: | Cheng, SM;Ho, YJ;Yu, SH;Liu, YF;Lin, YY;Huang, CY;Ou, HC;Huang, HL;Lee, SD |
| Keywords: | Aging;Apoptosis;Brain;D-galactose;Diosgenin;Survival |
| Date: | 2020 |
| Issue Date: | 2022-08-09T08:07:40Z (UTC)
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| Publisher: | WORLD SCIENTIFIC PUBL CO PTE LTD |
| ISSN: | 0192-415X |
| Abstract: | The purpose of this study was to evaluate the effects of diosgenin on the D-galactose-induced cerebral cortical widely dispersed apoptosis. Male 12-week-old Wistar rats were divided into four groups: Control (1 mg/kg/day of saline, i.p.), DDO (150 mg/kg/ day of D-galactose, i.p.), DD10, and DD50 (D-galactose+10 or 50 mg/kg/day of diosgenin orally). After eight weeks, histopathological analysis, positive TUNEL and Western blotting assays were performed on the excised cerebral cortex from all four groups. The TUNEL-positive apoptotic cells, the components of Fas pathway (Fas, FADD, active caspase-8 and active caspase-3), and mitochondria pathway (t-Bid, Bax, cytochrome c, active caspase-9 and active caspase-3) were increased in the DDO group compared with the control group, whereas they were decreased in the DD50 group. The components of survival pathway (p-Bad, Bcl-2, Bcl-xL, IGF-1, p-PI3K and p-AKT) were increased in the DD50 group compared to the control group, whereas the levels of Bcl-xL, p-PI3K, and p-AKT were also compensatorily increased in the DDO group compared to the control group. Taken together, diosgenin suppressed D-galactose-induced neuronal Fas-dependent and mitochondria-dependent apoptotic pathways and enhanced the Bcl-2 family associated pro-survival and IGF-1-PI3K-AKT survival pathways, which might provide neuroprotective effects of diosgenin for prevention of the D-galactose-induced aging brain. |
| URI: | http://dx.doi.org/10.1142/S0192415X20500202
https://www.webofscience.com/wos/woscc/full-record/WOS:000531553500008
https://ir.csmu.edu.tw:8080/handle/310902500/24766 |
| Relation: | AMERICAN JOURNAL OF CHINESE MEDICINE ,2020 ,v48 ,issue 2 ,p391-406 |
| Appears in Collections: | [中山醫學大學研究成果] 期刊論文
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