Salvianolic acid A suppresses MMP-2 expression and restrains cancer cell invasion through ERK signaling in human nasopharyngeal carcinoma

doi:10.1016/j.jep.2020.112601

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/24732

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/24732

Title:	Salvianolic acid A suppresses MMP-2 expression and restrains cancer cell invasion through ERK signaling in human nasopharyngeal carcinoma
Authors:	Chuang, CY;Ho, YC;Lin, CW;Yang, WE;Yu, YL;Tsai, MC;Yang, SF;Su, SC
Keywords:	Salvianolic acid A;Nasopharyngeal carcinoma;MMP-2;Invasion;Migration
Date:	2020
Issue Date:	2022-08-09T08:07:07Z (UTC)
Publisher:	ELSEVIER IRELAND LTD
ISSN:	0378-8741
Abstract:	Ethnopharmacological relevance: Salvia miltiorrhiza Bunge, as known as Danshen, has used for the prevention and treatment of cardiovascular diseases clinically and anti-cancer activities. Salvianolic acid A (SAA), one of the most abundant ingredients, hydrophilic derivatives of Salvia miltiorrhiza Bunge, exerts a variety of pharmacological actions, such as anti-oxidative, anti-inflammatory and anti-cancer activities. However, the impact of SAA on nasopharyngeal carcinoma (NPC) invasion and metastasis remains unexplored. Aim of the study: To investigate the potential of SAA to prevent migration and invasion on NPC cell. Materials and methods: MTT assay and Boyden chamber assay were performed to determine cell proliferation, migration and invasion abilities, respectively. The activity and protein expression of matrix metalloproteinase-2 (MMP-2) were determined by gelatin zymography and western blotting. Results: Here, we showed that SAA considerably suppressed the migrative and invasive activity of human NPC cells but not rendered cytotoxicity. In SAA-treated NPC cells, the activity and expression of matrix metalloproteinase-2 (MMP-2), a key regulator of cancer cell invasion, were reduced. Additionally, the presence of high concentrations of SAA dramatically abolished the activation of focal adhesion kinase (FAK) and moderately inhibited the phosphorylation of Src and ERK in NPC cells. Conclusions: Our results demonstrated that SAA inhibited the migration and invasion of NPC cells, accompanied by downregulation of MMP-2 and inactivation of FAK, Src, and ERK pathways. These findings indicate a usefulness of SAA on restraining NPC invasion and metastasis.
URI:	http://dx.doi.org/10.1016/j.jep.2020.112601 https://www.webofscience.com/wos/woscc/full-record/WOS:000518870800025 https://ir.csmu.edu.tw:8080/handle/310902500/24732
Relation:	JOURNAL OF ETHNOPHARMACOLOGY ,2020 ,v252
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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