Toxic Effects of Urethane Dimethacrylate on Macrophages Through Caspase Activation, Mitochondrial Dysfunction, and Reactive Oxygen Species Generation

doi:10.3390/polym12061398

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/24728

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/24728

Title:	Toxic Effects of Urethane Dimethacrylate on Macrophages Through Caspase Activation, Mitochondrial Dysfunction, and Reactive Oxygen Species Generation
Authors:	Chang, CY;Chiang, CY;Chiang, YW;Lee, MW;Lee, CY;Chen, HY;Lin, HW;Kuan, YH
Keywords:	UDMA;macrophage;genotoxicity;apoptosis;ROS generation;caspase activation
Date:	2020
Issue Date:	2022-08-09T08:07:03Z (UTC)
Publisher:	MDPI
Abstract:	Urethane dimethacrylate (UDMA) is a dimethacrylate-based resin monomer that can react with other related monomers and inorganic particles, causing hydrophobic polymerization through cross-linking upon light activation. UDMA polymers are commonly used for the reconstruction and reinforcement of teeth and bones. UDMA can become unbound and be released from light-cured polymer resins. Thus far, no evidence exists on the toxic effects of UDMA and its related working mechanisms for macrophages. Therefore, in the present study, we investigated the cytotoxicity, mode of cell death, DNA damage, caspase activities, mitochondrial dysfunction, and reactive oxygen species (ROS) generation in RAW264.7 macrophages treated with UDMA using the lactate dehydrogenase (LDH) assay kit, Annexin V-FITC and PI assays, micronucleus formation and comet assay, caspase fluorometric assay, JC-1 assay, and 2MODIFIER LETTER PRIME,7MODIFIER LETTER PRIME-dichlorofluorescin diacetate (DCFH-DA) assay, respectively. Our results show that UDMA induced cytotoxicity; apoptosis and necrosis; genotoxicity, which is also called DNA damage; increased caspase-3, -8, and -9 activities; mitochondrial dysfunction; and intracellular ROS generation in a concentration-dependent manner in RAW264.7 macrophages. Thus, based on the observed inhibited concentration parallel trends, we concluded that UDMA induces toxic effects in macrophages. Furthermore, UDMA-induced intracellular ROS generation, cytotoxicity, and DNA damage were reduced by N-acetyl-L-cysteine.
URI:	http://dx.doi.org/10.3390/polym12061398 https://www.webofscience.com/wos/woscc/full-record/WOS:000553551700001 https://ir.csmu.edu.tw:8080/handle/310902500/24728
Relation:	POLYMERS ,2020 ,v12 ,issue 6
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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