Extract Derived from Cedrus atlantica Acts as an Antitumor Agent on Hepatocellular Carcinoma Growth In Vitro and In Vivo

doi:10.3390/molecules25204608

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/24670

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/24670

Title:	Extract Derived from Cedrus atlantica Acts as an Antitumor Agent on Hepatocellular Carcinoma Growth In Vitro and In Vivo
Authors:	Huang, XF;Chang, KF;Lee, SC;Sheu, GT;Li, CY;Weng, JC;Hsiao, CY;Tsai, NM
Keywords:	hepatocellular carcinoma (HCC);Cedrus atlantica extract (CAt extract);cell cycle;apoptosis
Date:	2020
Issue Date:	2022-08-09T08:06:06Z (UTC)
Publisher:	MDPI
Abstract:	Cedrus atlantica is widely used in herbal medicine. However, the anti-cancer activity of C. atlantica extract (CAt extract) has not been clarified in hepatocellular carcinoma. In the study, we elucidated the anti-hepatoma capacity of CAt extract on HCC in vitro and in vivo. To explore the anti-hepatoma mechanisms of the CAt extract in vitro, HCC and normal cells were treated with the CAt extract, which showed marked inhibitory effects on HCC cells in a dose-dependent manner; in contrast, the CAt extract treatment was less cytotoxic to normal cells. In addition, our results indicate that the CAt extract induced apoptosis via caspase-dependent and independent apoptosis pathways. Furthermore, the CAt extract inhibited HCC tumor cell growth by restraining cell cycle progression, and it reduced the signaling of the AKT, ERK1/2, and p38 pathways. In the xenograft model, the CAt extract suppressed HCC tumor cell growth and prolonged lifespan by inhibiting PCNA protein expression, repressing part of the VEGF-induced autocrine pathway, and triggering strong expression of cleaved caspase-3, which contributed to cell apoptosis. Moreover, the CAt extract did not induce any obvious changes in pathological morphology or body weight, suggesting it had no toxicity. CAt extract exerted anti-tumor effects on HCC in vitro and in vivo. Thus, CAt extract could be used as a potential anti-cancer therapeutic agent against HCC.
URI:	http://dx.doi.org/10.3390/molecules25204608 https://www.webofscience.com/wos/woscc/full-record/WOS:000585707200001 https://ir.csmu.edu.tw:8080/handle/310902500/24670
Relation:	MOLECULES ,2020 ,v25 ,issue 20
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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