The sex-specific association of phthalate exposure with DNA methylation and characteristics of body fat in children

doi:10.1016/j.scitotenv.2020.139833

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/24665

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/24665

Title:	The sex-specific association of phthalate exposure with DNA methylation and characteristics of body fat in children
Authors:	Chang, CH;Chen, CF;Tsai, YA;Wang, SL;Huang, PC;Chen, BH;Wu, MT;Chen, CC;Hsiung, CA;Chen, ML
Keywords:	Phthalates;Children;Body fat;DNA methylation;POLG
Date:	2020
Issue Date:	2022-08-09T08:06:02Z (UTC)
Publisher:	ELSEVIER
ISSN:	0048-9697
Abstract:	The present study assessed the association between phthalate exposure and mitochondrial DNA (mtDNA) poly-merase gamma (POLG) methylation along with the potential effect on the characteristics of body fat in children. A total of 152 children were enrolled. The urinary concentrations of phthalate metabolites were measured using ultraperformance liquid chromatography-tandem mass spectrometry. Genomic DNA was extracted from the buffy coat, and bisulfite-treated DNA was subjected to a pyrosequencing assay. In total, 17 CpG sites in the exon 2 region of POLG were included in the analysis. A multivariable regression model was applied to determine whether characteristics of body fat were associated with phthalate exposure and methylation of POLG. After adjustment for covariates, male children with a ten-fold increase in mono-methyl phthalate (MMP) or mono-benzyl phthalate (MBzP) concentrations had significantly higher measurements for total body fat (MMP: beta = 6.47%; MBzP: beta = 3.54%), and trunk fat (MMP: beta = 6.67%; MBzP: beta = 3.90%). Male children who had hypermethylation at the 2nd CpG site in exon 2 of POLG also had high measurements for BMI (beta = 1.66 kg/m(2)), waist (beta = 4.49 cm) and hip (beta = 4.81 cm) circumference, total body fat (beta = 5.48%), and trunk fat (beta = 6.21%). A dose-response relationship existed between methylation at the 2nd CpG site in exon 2 of POLG and characteristics of body fat (p for trend <0.01). This study suggested that male children who are exposed to phthalic acid esters have high body weight, BMI, and body and trunk fat percentages. Methylation of the exon 2 region of POLG is a possible mechanism behind the causal effect of endocrine-disrupting substances. (C) 2020 Published by Elsevier B.V.
URI:	http://dx.doi.org/10.1016/j.scitotenv.2020.139833 https://www.webofscience.com/wos/woscc/full-record/WOS:000553713200004 https://ir.csmu.edu.tw:8080/handle/310902500/24665
Relation:	SCIENCE OF THE TOTAL ENVIRONMENT ,2020 ,v737
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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