Water-ExtractedGanoderma lucidumInduces Apoptosis and S-Phase Arrest via Cyclin-CDK2 Pathway in Glioblastoma Cells

doi:10.3390/molecules25163585

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/24629

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/24629

Title:	Water-ExtractedGanoderma lucidumInduces Apoptosis and S-Phase Arrest via Cyclin-CDK2 Pathway in Glioblastoma Cells
Authors:	Cheng, AY;Chien, YC;Lee, HC;Hsieh, YH;Yu, YL
Keywords:	glioblastoma;Ganoderma lucidum;apoptosis;S phase arrest;CDK2
Date:	2020
Issue Date:	2022-08-09T08:05:27Z (UTC)
Publisher:	MDPI
Abstract:	Glioblastoma is one of the most common and most aggressive brain cancers. The current treatment is mainly surgery, chemotherapy, and radiation therapy, but the results are not satisfactory.Ganoderma lucidum(G. lucidum), also called Lingzhi, is a medicinal mushroom that has been used as a therapeutic agent for the treatment of numerous diseases, including cancer. However, whether it is effective for treating cancer is still unclear. In the present study, the anti-tumor effect of a water extract ofG. lucidumwas investigated using brain tumor cells. We used an analysis of cell viability, flow cytometry, the IncuCyte live-cell analysis system, and Western blotting to study its effects. The water extract fromG. luciduminhibited cell proliferation in a dose- and time-dependent manner, and it induced mitochondria-mediated apoptosis and cell cycle arrest at S phase via the cyclin-CDK2 pathway in human brain tumor cells. In addition, theG. lucidumextract significantly inhibited cell migration and mesenchymal marker expression based on the IncuCyte live-cell assay and qRT-PCR analysis. In summary, these anti-tumor effects in brain tumor cells suggest thatG. lucidummay be useful for treating brain tumors.
URI:	http://dx.doi.org/10.3390/molecules25163585 https://www.webofscience.com/wos/woscc/full-record/WOS:000578864800001 https://ir.csmu.edu.tw:8080/handle/310902500/24629
Relation:	MOLECULES ,2020 ,v25 ,issue 16
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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