Mulberry fruits extracts induce apoptosis and autophagy of liver cancer cell and prevent hepatocarcinogenesis in vivo

doi:10.1016/j.jfda.2019.06.002

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/24597

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/24597

Title:	Mulberry fruits extracts induce apoptosis and autophagy of liver cancer cell and prevent hepatocarcinogenesis in vivo
Authors:	Cheng, KC;Wang, CJ;Chang, YC;Hung, TW;Lai, CJ;Kuo, CW;Huang, HP
Keywords:	Apoptosis;Autophagy;Hepatocellular carcinoma;Mulberry water extracts;Polyphenol
Date:	2020
Issue Date:	2022-08-09T08:04:57Z (UTC)
Publisher:	DIGITAL COMMONS BEPRESS
ISSN:	1021-9498
Abstract:	Hepatocellular carcinoma (HCC) is one of the most common malignancies in Taiwan. Many risks factors induce liver chronic inflammation, fibrosis, cirrhosis, and hepatocellular carcinoma. Mulberry fruits containing polyphenols to remove free radicals and mitigate inflammation has been reported to not only against gastric cancer, melanoma and leukemia but also prevent liver injury induced by alcohol or CCl4 in previous researches. The aim of this study is to examine whether Mulberry could inhibit hepatocarcinogenesis. In animal experiment, diethylnitrosamine (DEN) was used to induce hepatic tumorgenesis. After injecting DEN, the rats treated with mulberry water extracts (MWE) had less and smaller tumor than others without MWE. Moreover, MWE reduced the serum ALT and AST, HCC marker, cleavage caspases, Ser-15-p53 and Ser-46-p53 induced by DEN. Further, we observed that mulberry polyphenol extracts (MPE) inhibited the cell growth of HepG2 cell and Hep3B cell. By using flow cytometry and western blotting methods, MPE induced HepG2 cell apoptosis by increase subG1 cells and the elevated expression of caspase-3/8/9. Instead of apoptosis, MPE caused Hep3B cells autophagy by inhibiting Akt and mTOR phosphorylation. Comprehensively, mulberry extracts has a potential to be a health supplement to prevent hepatocarcinogenesis in the future.
URI:	http://dx.doi.org/10.1016/j.jfda.2019.06.002 https://www.webofscience.com/wos/woscc/full-record/WOS:000504756400006 https://ir.csmu.edu.tw:8080/handle/310902500/24597
Relation:	JOURNAL OF FOOD AND DRUG ANALYSIS ,2020 ,v28 ,issue 1 ,p84-93
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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