Aberrant DNA methylation levels of the formyl peptide receptor 1/2/3 genes are associated with obstructive sleep apnea and its clinical phenotypes

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题名:	Aberrant DNA methylation levels of the formyl peptide receptor 1/2/3 genes are associated with obstructive sleep apnea and its clinical phenotypes
作者:	Chen, YC;Huang, KT;Su, MC;Hsu, PY;Chin, CH;Lin, IC;Liou, CW;Wang, TY;Lin, YY;Hsiao, CC;Lin, MC
关键词:	Obstructive sleep apnea;formyl peptide receptor;DNA methylation;intermittent hypoxia with re-oxygenation
日期:	2020
上传时间:	2022-08-09T08:03:27Z (UTC)
出版者:	E-CENTURY PUBLISHING CORP
ISSN:	1943-8141
摘要:	Background: FPR1 over-expression and insufficiency of FPR2 and FPR3 are associated with disease severity of obstructive sleep apnea (OSA). We hypothesized that epigenetic modification of the FPR1/2/3 genes may underlie intermittent hypoxia with re-oxygenation (IHR) injury in OSA. Methods: DNA methylation levels over 17 CpG sites of the FPR1/2/3 genes and their gene expression levels in the peripheral blood mononuclear cells were determined in 40 treatment-naive OSA patients, 12 severe OSA patients under long-term continuous positive airway pressure treatment, 16 primary snoring (PS) subjects, and 10 healthy non-snorers (HS). Results: Both-524 and-264 CpG sites of the FPR1 gene were hypomethylated in treatment-naive OSA versus HS, while-264 CpG site methylation level was negatively correlated with FPR1/FPR3 gene expression ratio and associated with prevalent diabetes mellitus. Both +8802 and +8845 CpG sites of the FPR2 gene were hypermethylated in treatment-naive OSA versus HS, while hypermethylated +9132 and +9150 CpG sites were both associated with prevalent hypertension. FPR3 gene expression and DNA methylation levels over-842/-516 CpG sites of the FPR3 gene were both decreased in treatment-naive OSA versus HS, while hypermethylated-429 CpG site was associated with elevated serum C-reactive protein level. In vitro IHR stimuli in human monocytic THP-1 cells resulted in gene promoter hypomethylation-mediated FPR1 over-expression, increased production of reactive oxygen species, and increased cell apoptosis, which could be reversed with re-methylation agent, folic acid, treatment. Conclusions: Aberrant DNA methylation patterns of the FPR1/2/3 gene promoters contribute to disease severity and diabetes mellitus or cardiovascular disease in OSA patients, probably through regulating FPR1/2/3 gene expressions.
URI:	https://www.webofscience.com/wos/woscc/full-record/WOS:000548245700014 https://ir.csmu.edu.tw:8080/handle/310902500/24506
關聯:	AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH ,2020 ,v12 ,issue 6 ,p2521-2537
显示于类别:	[中山醫學大學研究成果] 期刊論文

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