Sesamin inhibits cervical cancer cell proliferation by promoting p53/PTEN-mediated apoptosis

doi:10.7150/ijms.48955

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/24455

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/24455

Title:	Sesamin inhibits cervical cancer cell proliferation by promoting p53/PTEN-mediated apoptosis
Authors:	Kuo, TN;Lin, CS;Li, GD;Kuo, CY;Kao, SH
Keywords:	Sesamin;Cervical cancer cell;p53;PTEN;PUMA;Apoptosis
Date:	2020
Issue Date:	2022-08-09T08:02:36Z (UTC)
Publisher:	IVYSPRING INT PUBL
ISSN:	1449-1907
Abstract:	Background: Sesamin is a major bioactive compound in sesame seeds and has various biological properties, including anti-inflammatory and anticancer activities. Here, we explored whether sesamin activates p53, which is widely inhibited in cervical cancer cells, thereby inducing p53-mediated apoptosis. Methods: Human HeLa and SiHa cervical cancer cells and normal Hs68 dermal cells were used as cell models. Cell proliferation, cell cycle distribution, and apoptosis were evaluated by the CCK-8 assay and flow cytometry using PI/Annexin V staining, respectively. Protein expression and phosphorylation were determined using western blotting. The involvement of p53 in the apoptotic cascade was assessed by a specific inhibitor. Results: Sesamin (75 and 150 mu M) clearly inhibited SiHa and HeLa cell proliferation in a dose-dependent fashion, but did not affect the proliferation of Hs68 cells. Meanwhile, sesamin increased the sub-G1 phase ratio and apoptosis, up to approximately 38.5% and 37.8%, respectively. Furthermore, sesamin induced p53 phosphorylation at serine-46 and serine-15 and upregulated the levels of PUMA, Bax, and PTEN, while inhibiting AKT phosphorylation at serine-473. Inhibition of p53 by pifithrin-alpha significantly reduced the levels of PUMA, Bax, and PTEN but restored AKT phosphorylation in SiHa cells exposed to sesamin. Pifithrin-alpha also reduced apoptosis and restored the proliferation of HeLa and SiHa cells exposed to sesamin. Conclusions: These findings indicate that sesamin inhibits cervical cancer cell proliferation, and its mechanism may be attributed to the induction of p53/PTEN-mediated apoptosis. This suggests that sesamin might be useful as an adjuvant in promoting anti-cervical cancer treatments.
URI:	http://dx.doi.org/10.7150/ijms.48955 https://www.webofscience.com/wos/woscc/full-record/WOS:000573070000008 https://ir.csmu.edu.tw:8080/handle/310902500/24455
Relation:	INTERNATIONAL JOURNAL OF MEDICAL SCIENCES ,2020 ,v17 ,issue 15 ,p2292-2298
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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