In vitro proliferation of human epidermal melanocytes biopsied from multiple anatomical sites

doi:10.1111/jocd.13348

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/24294

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/24294

Title:	In vitro proliferation of human epidermal melanocytes biopsied from multiple anatomical sites
Authors:	Yang, HJ;Yang, KC;Wang, YF;Yang, YT;Ko, JL
Keywords:	autologous transplantation;cell culture;cell expansion;melanocyte;vitiligo
Date:	2020
Issue Date:	2022-08-09T07:59:57Z (UTC)
Publisher:	WILEY
ISSN:	1473-2130
Abstract:	Background Vitiligo is an acquired disease that involves low pigment variation in the skin. The use of cultured melanocytes for treatment of recalcitrant vitiligo has become a well-established treatment modality. In vitro cultured melanocytes present an effective autologous transplantation treatment modality for vitiligo. The present study investigated the in vitro culture of epidermal melanocytes sampled from multiple body parts, as well as the differences in total propagation time. Methods Skin specimens were collected from 30 participants (14 males and 16 females) who supplied normal colored skin sections from various regions of their body, including the face, chest, abdomen, buttock, and extremities. Subsequently, all of these specimens were treated with an identical melanocyte purification and culturing process. Results Melanocytes from the face demonstrated the most rapid growth and longest total propagation time. Melanocytes from the buttock, abdomen, and extremities displayed similar results to one another, and melanocytes from the chest and back had the slowest growth and shortest total propagation time. Conclusion Selecting the most favorable site to obtain epidermal melanocytes will reduce the required quantity of skin and culturing time, and maximize the growth and total propagation time of melanocytes. Therefore, care should be exercised when selecting the region of skin when culturing epidermal melanocytes.
URI:	http://dx.doi.org/10.1111/jocd.13348 https://www.webofscience.com/wos/woscc/full-record/WOS:000517623000001 https://ir.csmu.edu.tw:8080/handle/310902500/24294
Relation:	JOURNAL OF COSMETIC DERMATOLOGY ,2020 ,v19 ,issue 11 ,p3077-3082
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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