Effects of Coronavirus Persistence on the Genome Structure and Subsequent Gene Expression, Pathogenicity and Adaptation Capability

doi:10.3390/cells9102322

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/24278

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/24278

Title:	Effects of Coronavirus Persistence on the Genome Structure and Subsequent Gene Expression, Pathogenicity and Adaptation Capability
Authors:	Lin, CH;Yang, CY;Wang, ML;Ou, SC;Lo, CY;Tsai, TL;Wu, HY
Keywords:	coronavirus;persistence;genome structure;gene expression;pathogenicity;virus fitness
Date:	2020
Issue Date:	2022-08-09T07:59:40Z (UTC)
Publisher:	MDPI
Abstract:	Coronaviruses are able to establish persistence. However, how coronaviruses react to persistence and whether the selected viruses have altered their characteristics remain unclear. In this study, we found that the persistent infection of bovine coronavirus (BCoV), which is in the same genus as SARS-COV-2, led to alterations of genome structure, attenuation of gene expression, and the synthesis of subgenomic mRNA (sgmRNA) with a previously unidentified pattern. Subsequent analyses revealed that the altered genome structures were associated with the attenuation of gene expression. In addition, the genome structure at the 5 ' terminus and the cellular environment during the persistence were responsible for the sgmRNA synthesis, solving the previously unanswered question regarding the selection of transcription regulatory sequence for synthesis of BCoV sgmRNA 12.7. Although the BCoV variants (BCoV-p95) selected under the persistence replicated efficiently in cells without persistent infection, its pathogenicity was still lower than that of wild-type (wt) BCoV. Furthermore, in comparison with wt BCoV, the variant BCoV-p95 was not able to efficiently adapt to the challenges of alternative environments, suggesting wt BCoV is genetically robust. We anticipate that the findings derived from this fundamental research can contribute to the disease control and treatments against coronavirus infection including SARS-CoV-2.
URI:	http://dx.doi.org/10.3390/cells9102322 https://www.webofscience.com/wos/woscc/full-record/WOS:000584115800001 https://ir.csmu.edu.tw:8080/handle/310902500/24278
Relation:	CELLS ,2020 ,v9 ,issue 10
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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