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https://ir.csmu.edu.tw:8080/ir/handle/310902500/24037
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题名: | Epidermal growth factor receptors siRNA-conjugated collagen modified gold nanoparticles for targeted imaging and therapy of lung cancer |
作者: | Yu, AYH;Fu, RH;Hsu, SH;Chiu, CF;Fang, WH;Yeh, CA;Tang, CM;Hsieh, HH;Hung, HS |
关键词: | Epidermal growth factor receptor;Gold nanoparticle (AuNP);Small-interfering RNA (siRNA);Lung cancer |
日期: | 2021 |
上传时间: | 2022-08-05T09:46:50Z (UTC)
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出版者: | ELSEVIER |
ISSN: | 2590-0498 |
摘要: | Epidermal growth factor receptor (EGFR), a critical factor promotes lung cancer cell proliferation and survival. Knockdown of EGFR expression thus promise beating lung cancer clinically. Functionalized gold nanoparticles may serve an effective vehicle carrying theranostic bio-active materials. Herein, physically gold nanoparticles were fabricated with biocompatible collagen to improve siRNA loading capacity carrying EGFR siRNA to treat lung cancer. Physic-chemical properties were comprehensively characterized for the collagen gold nanoparticle (C-Au), and with EGFR siRNA conjugation, C-Au-EGFRsi namely. Issues of biocompatibility were addressed. Interestingly, C-Au appeared more biocompatible to normal airway epithelial cells (BEAS-2B) than to cancer cells (A549) in terms of ROS production, cell cycle behavior, and cell growth influence. The C-Au demonstrated comparable or even more efficient, compared with lipofetamine, in carrying siRNA to knockdown EGFR of A549 cells. Endocytosis mediated cell entry for the collagen gold nanoparticles, and endosome-lysosomal pathway involved transporting and metabolizing these nanoparticles. In xenograft mice model, substantial tumor suppression effects were observed treating with C-Au-EGFRsi, in which tumor weight reduced 30% for lipofetamine carrier, and down to 70% for C-Au carrier. Particularly, overall survival rate was improved for both treatment groups with lipofetamine and C-Au carrier, respectively. (c) 2021 Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/). |
URI: | http://dx.doi.org/10.1016/j.mtadv.2021.100191 https://www.webofscience.com/wos/woscc/full-record/WOS:000744197600005 https://ir.csmu.edu.tw:8080/handle/310902500/24037 |
關聯: | MATERIALS TODAY ADVANCES ,2021,v12 |
显示于类别: | [中山醫學大學研究成果] 期刊論文
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