Groundwater near refinery and natural gas plants often contain elevated concentrations of toxic sulfolane. Studies on any concentration of sulfolane are limited. Column experiment was conducted to investigate the effects of adding a low dose of H2O2 and nutrient on bioremediation. Vibrio fischeri light inhibition test was used evaluate the toxicity of effluents. The continuous column experiment conditions were sulfolane at 100 mg L-1, dissolved oxygen at 7 mg L-1, absence of phosphorus, and very short hydraulic retention time (7.9 h). A low dose of H2O2 (5.88 mM) enhanced the sulfolane (27.1%) and COD removal (11.8%) in comparison with the control set. Adding nutrient increased bicinchoninic acid protein assay levels, sulfolane removal (99.6%) and COD removal (80.3%). Addition of both H2O2 and nutrient further improved COD removal (90.3%) and COD/sulfolane ratio (0.90) and toxicity removal (Vibrio fischeri light inhibition ratio < 1%). Batch experiment indicated the degraders tolerated sulfolane up to 400 mg L-1. The DGGE method and dendrogram analysis were utilized to investigate the changes of degrader community structure.
