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https://ir.csmu.edu.tw:8080/ir/handle/310902500/23944
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Title: | Fine particulate matter PM2.5 generated by building demolition increases the malignancy of breast cancer MDA-MB-231 cells |
Authors: | Cheng, CW;Sheu, GT;Chou, JS;Wang, PH;Cheng, YC;Lai, CY |
Keywords: | Demolition;PM2.5;Heavy metals;Breast cancer;MDA-MB-231;Malignancy |
Date: | 2021 |
Issue Date: | 2022-08-05T09:45:21Z (UTC)
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Publisher: | PERGAMON-ELSEVIER SCIENCE LTD |
ISSN: | 0045-6535 |
Abstract: | Objectives: This study investigates the effects of water-extracted PM2.5 on a triple-negative breast cancer (TNBC) cell line, MDA-MB-231, by sampling suspended particulates around a building demolition site. Methods: PM2.5 particles were obtained using a high-flow TISCH sampler. Water-soluble PM2.5 were extracted by an ultrasonic oscillator and then freeze-dried. The heavy metal components of soluble PM2.5 was analyzed by ICP-MS. Cell viability was evaluated by MTT assay for cells that were exposed to PM2.5 (200, 400 and 600 ug/mL). Wound healing and transwell cell migration and invasion assays were used to measure cell motility and the invasiveness of cancer cells that had been exposed to PM2.5 into a chemo-attractant substance. Interrelated mechanisms of cancer malignancy were analyzed by Western blot analysis. Results: Nearby PM2.5 concentrations increased significantly during the deconstruction of buildings, and the Cd, Cu, Pb, Zn and Cr contents of soluble PM2.5 also significantly increased. Following exposure to PM2.5 , the survival rate of breast cancer cells was significantly higher than that of the control group. Soluble PM2.5 -treated cells had a higher migration capacity. The signaling pathway of FAK/PI3K/AKT proteins was more activated in PM2.5 -treated cells than the control group. Increased levels of Aurora B and Bcl-2 were associated with cell proliferation. Elevated levels of cathepsins D, beta-catenin, N-cadherin, vimentin and MMP-9 were associated with breast cancer cell metastasis. Conclusion: Soluble PM2.5 from building demolition may promote/progress in surviving TNBC cells, increasing the malignancy of breast cancer. This study offered evidence of a link between demolition PM2.5 and cancer progression. (C) 2020 Published by Elsevier Ltd. |
URI: | http://dx.doi.org/10.1016/j.chemosphere.2020.129028 https://www.webofscience.com/wos/woscc/full-record/WOS:000603465000024 https://ir.csmu.edu.tw:8080/handle/310902500/23944 |
Relation: | CHEMOSPHERE ,2021,v265 |
Appears in Collections: | [中山醫學大學研究成果] 期刊論文
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