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https://ir.csmu.edu.tw:8080/ir/handle/310902500/23783
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| 题名: | Cetyltrimethylammonium Bromide Disrupts Mesenchymal Characteristics of Human Tongue Squamous Cell Carcinoma SCC4 Cells Through Modulating Canonical TGF-beta/Smad/miR-181b/TIMP3 Signaling Pathway |
| 作者: | Yue, CH;Chen, CH;Pan, YR;Chen, YP;Huang, FM;Lee, CJ |
| 关键词: | Cetyltrimethylammonium bromide;CTAB;tongue squamous cell carcinoma;TSCC;epithelial-to-mesenchymal transition;EMT;transforming growth factor-βTGF-&beta |
| 日期: | 2021 |
| 上传时间: | 2022-08-05T09:42:49Z (UTC)
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| 出版者: | INT INST ANTICANCER RESEARCH |
| ISSN: | 0250-7005 |
| 摘要: | Background/Aim: This study investigated the anti metastatic effects of cetyltrimethylammonium bromide (CTAB) on tongue squamous cell carcinoma (TSCC) SCC4 cells. Materials and Methods: Cell morphology, viability, cell cycle distribution, adhesion, migration, invasion and the expression levels of associated proteins were examined using microscopy, WST-1, wound-healing, Boyden chamber assays, and western blotting, respectively. Results: CTAB significantly affected SCC4 cell morphology from spindle-shaped to cobblestone-shaped and resulted in loss of adherence. CTAB significantly inhibited cell adhesion, migration, and invasion of SCC4 cells, independent of cell viability. CTAB reduced expression of matrix metalloproteinases (MMPs) such as MMP3, MMP7, and MMP14 in a concentration-dependent manner, while it increased expression of tissue inhibitors of metalloproteinase 3 (TIMP3). In addition, CTAB reduced the phosphorylation of mothers against decapentaplegic homolog 2/3 (Smad2/3) proteins, which mediated CTAB-inhibited migration and invasion in SCC4 cells. These effects were reversed by TGF-beta 1. Conclusion: CTAB attenuates the mesenchymal characteristics through upregulation of TIMP3 by inhibiting the canonical TGF-beta/Smad/miR-181b/TIMP3 signaling involved in extracellular matrix remodeling in SCC4 cells and might be a promising anti-metastatic therapeutic agent for TSCC. |
| URI: | http://dx.doi.org/10.21873/anticanres.15429
https://www.webofscience.com/wos/woscc/full-record/WOS:000725492100024
https://ir.csmu.edu.tw:8080/handle/310902500/23783 |
| 關聯: | ANTICANCER RESEARCH ,2021,v41,issue 12, P6095-6104 |
| 显示于类别: | [中山醫學大學研究成果] 期刊論文
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