Anti-inflammatory effects of Flos Lonicerae Japonicae Water Extract are regulated by the STAT/NF-kappa B pathway and HO-1 in Virus-infected RAW264.7 cells

doi:10.7150/ijms.56198

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/23762

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/23762

Title:	Anti-inflammatory effects of Flos Lonicerae Japonicae Water Extract are regulated by the STAT/NF-kappa B pathway and HO-1 in Virus-infected RAW264.7 cells
Authors:	Lin, HW;Lee, YJ;Yang, DJ;Hsieh, MC;Chen, CC;Hsu, WL;Chang, YY;Liu, CW
Keywords:	Flos Lonicerae Japonicae water extract (FLJWE);pseudorabies virus (PRV);antiviral inflammatory;heme oxygenase-1 (HO-1)
Date:	2021
Issue Date:	2022-08-05T09:42:27Z (UTC)
Publisher:	IVYSPRING INT PUBL
ISSN:	1449-1907
Abstract:	This study examined the effect of the Flos Lonicerae Japonicae water extract (FLJWE), chlorogenic acid, and luteolin on pseudorabies virus (PRV)-induced inflammation in RAW264.7 cells and elucidated related molecular mechanisms. The results revealed that FLJWE and luteolin, but not chlorogenic acid, inhibited the production of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and inflammatory cytokines in PRV-infected RAW 264.7 cells. We found that the FLJWE and luteolin suppressed nuclear factor (NF)-kappa B activation by inhibiting the phosphorylation of signal transducer and activator of transcription 1 and 3 (STAT1 and STAT3, respectively). Moreover, the FLJWE significantly upregulated the expression of pNrf2 and its downstream target gene heme oxygenase-1 (HO-1). Our data indicated that FLJWE and luteolin reduced the expression of proinflammatory mediators and inflammatory cytokines, such as COX-2 and iNOS, through the suppression of the JAK/STAT1/3-dependent NF-kappa B pathway and the induction of HO-1 expression in PRV-infected RAW264.7 cells. The findings indicate that the FLJWE can be used as a potential antiviral agent.
URI:	http://dx.doi.org/10.7150/ijms.56198 https://www.webofscience.com/wos/woscc/full-record/WOS:000643794500005 https://ir.csmu.edu.tw:8080/handle/310902500/23762
Relation:	INTERNATIONAL JOURNAL OF MEDICAL SCIENCES ,2021,v18,issue 11, P2285-2293
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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