Silencing periostin inhibits myofibroblast transdifferentiation of fibrotic buccal mucosal fibroblasts

doi:10.1016/j.jfma.2021.04.008

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/23735

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/23735

Title:	Silencing periostin inhibits myofibroblast transdifferentiation of fibrotic buccal mucosal fibroblasts
Authors:	Su, JY;Yu, CC;Peng, CY;Liao, YW;Hsieh, PL;Yang, LC;Yu, CH;Chou, MY
Keywords:	Oral submucous fibrosis;Periostin;Buccal mucosal fibroblasts
Date:	2021
Issue Date:	2022-08-05T09:42:01Z (UTC)
Publisher:	ELSEVIER TAIWAN
ISSN:	0929-6646
Abstract:	Background/Purpose: Oral submucous fibrosis (OSF) a well-recognized oral premalignant disor-der. Several studies have demonstrated that periostin, a matricellular protein, is involved in the development and pathogenesis of fibrosis diseases. Nevertheless, the contribution of peri-ostin in OSF remains to be uncovered. The purpose of the study was to illustrate the functional role of periostin involved in OSF pathogenesis. Methods: RNA-sequencing was employed to screen for differentially expressed genes in normal and OSF tissues. Validation of the upregulation of periostin in OSF specimens and fibrotic buccal mucosal fibroblasts (fBMFs) was conducted by qRT-PCR. The correlation of the gene expression of periostin and various fibrosis markers was analyzed. In addition, the functional role of periostin in myofibroblast features was tested using collagen gel contraction and trans-well migration assays. Results: We observed overexpression of periostin in OSF specimens using RNA-sequencing and confirmed its upregulation in OSF tissues and patient-derived fBMFs. Besides, there was a pos-itive relationship between the expression of periostin and several fibrosis-associated markers, including ACTA2 (alpha-smooth muscle actin; alpha-SMA), COL1A1 (type 1 collagen alpha 1 chain), TGFB1 (TGF-beta 1), and FN1 (fibronectin). Furthermore, we examined the effect of silencing periostin on the maintenance of myofibroblast characteristics and showed that knockdown of periostin sup-pressed the expression of alpha-SMA. Also, inhibition of periostin markedly downregulated the myofibroblast activities (collagen gel contraction and migration capacities). Conclusion: Our results indicate the aberrant expression of periostin in OSF tissues and myofi-broblasts. Moreover, the expression of periostin is positively associated with fibrosis markers, and repression of periostin may be a promising direction to alleviate the progression of OSF. Copyright (C) 2021, Formosan Medical Association. Published by Elsevier Taiwan LLC.
URI:	http://dx.doi.org/10.1016/j.jfma.2021.04.008 https://www.webofscience.com/wos/woscc/full-record/WOS:000709764100011 https://ir.csmu.edu.tw:8080/handle/310902500/23735
Relation:	JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION ,2021,v120,issue 11, P2010-2015
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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