中山醫學大學機構典藏 CSMUIR:Item 310902500/23570
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    題名: The effect of human platelet lysate on corneal nerve regeneration
    作者: Huang, CT;Chu, HS;Hung, KC;Chen, LW;Chen, MY;Hu, FR;Chen, WL
    關鍵詞: platelet lysate;corneal nerve regeneration;neurotrophic keratopathy
    日期: 2021
    上傳時間: 2022-08-05T09:39:26Z (UTC)
    出版者: BMJ PUBLISHING GROUP
    ISSN: 0007-1161
    摘要: Aim This study aimed to test whether human platelet lysate (HPL) has neurotrophic ability for corneal nerve regeneration. Methods We measured the neurotrophic factors in human peripheral serum (HPS) and two commercially available HPLs, UltraGRO and PLTMax. In vitro, we compared the growth rates, neuronal differentiation and immunostaining of neuron markers in mouse neuroblastoma cell line (Neuro-2a) and primary culture of mouse trigeminal ganglion cells that were cultivated in different concentrations of fetal bovine serum, HPS and HPL. In vivo, we created corneal wounds on Sprague Dawley rats with a rotating burr and evaluated the effects of topical HPL on wound healing and corneal nerve regeneration by in vivo confocal microscopy and corneal aesthesiometry. Results HPLs had significantly higher concentrations of various neurotrophic factors compared with HPS (p<0.05). In Neuro-2a cells, 3% HPL was better at promoting neuronal growth and differentiation compared with HPS at the same concentration. HPL was also found to have superior neurotrophic effects compared with HPS in primary cultures of mouse trigeminal ganglion cells. In vivo, HPL-treated eyes had better corneal epithelial wound healing rate, nerve regeneration length and corneal touch threshold compared with eyes treated with artificial tears (p<0.05). Conclusion HPL has significantly higher concentrations of neurotrophic factors compared with HPS. It showed not only in vitro but also in vivo corneal neurotrophic abilities. Our results suggest that HPL may have a potential role in the treatment of diseases related to corneal nerve damage or degeneration.
    URI: http://dx.doi.org/10.1136/bjophthalmol-2019-314408
    https://www.webofscience.com/wos/woscc/full-record/WOS:000654243300024
    https://ir.csmu.edu.tw:8080/handle/310902500/23570
    關聯: BRITISH JOURNAL OF OPHTHALMOLOGY ,2021,v105,issue 6, P884-890
    顯示於類別:[中山醫學大學研究成果] 期刊論文

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