Acrylamide Induces Mitophagy and Alters Macrophage Phenotype via Reactive Oxygen Species Generation

doi:10.3390/ijms22041683

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/23496

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/23496

Title:	Acrylamide Induces Mitophagy and Alters Macrophage Phenotype via Reactive Oxygen Species Generation
Authors:	Hung, CH;Lin, YC;Tsai, YG;Lin, YC;Kuo, CH;Tsai, ML;Kuo, CH;Liao, WT
Keywords:	acrylamide;reactive oxygen species (ROS);mitophagy;epigenetics;macrophage
Date:	2021
Issue Date:	2022-08-05T09:38:12Z (UTC)
Publisher:	MDPI
Abstract:	Acrylamide is a readily exposed toxic organic compound due to its formation in many carbohydrate rich foods that are cooked at high temperatures. Excessive production of reactive oxygen species (ROS), which is an important factor for mitophagy, has been reported to lead to airway inflammation, hyper-responsiveness, and remodeling. Epigenetic regulation is an important modification affecting gene transcription. In this study, the effects of acrylamide on ROS productions and mitophagy were investigated. The human monocytic cell line THP-1 was treated with acrylamide, and ROS productions were investigated by flow cytometry. The mitochondrial and epigenetic involvement was evaluated by quantitative real-time PCR. Histone modifications were examined by chromatin immunoprecipitation assays. Mitophagy was detected by Western blotting and confocal laser microscopy. Acrylamide promoted mitochondria-specific ROS generation in macrophages. The gene expression of mitochondrial respiratory chain complex II SDHA was increased under acrylamide treatment. Acrylamide induced histone H3K4 and H3K36 tri-methylation in an SDHA promoter and increased mitophagy-related PINK1 expression, which promoted a M2-like phenotypic switch with increase TGF-beta and CCL2 levels in THP-1 cells. In conclusion, acrylamide induced ROS production through histone tri-methylation in an SDHA promoter and further increased the expression of mitophagy-related PINK-1, which was associated with a macrophage M2 polarization shift.
URI:	http://dx.doi.org/10.3390/ijms22041683 https://www.webofscience.com/wos/woscc/full-record/WOS:000623821800001 https://ir.csmu.edu.tw:8080/handle/310902500/23496
Relation:	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ,2021,v22,issue 4
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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