Bisphenol A induced apoptosis via oxidative stress generation involved Nrf2/HO-1 pathway and mitochondrial dependent pathways in human retinal pigment epithelium (ARPE-19) cells

doi:10.1002/tox.23384

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/23372

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题名:	Bisphenol A induced apoptosis via oxidative stress generation involved Nrf2/HO-1 pathway and mitochondrial dependent pathways in human retinal pigment epithelium (ARPE-19) cells
作者:	Chiang, YW;Su, CH;Sun, HY;Chen, SP;Chen, CJ;Chen, WY;Chang, CC;Chen, CM;Kuan, YH
关键词:	apoptosis;ARPE-19 cells;bisphenol A;mitochondrial dysfunction;Nrf2;HO-1 pathway
日期:	2021
上传时间:	2022-08-05T09:36:13Z (UTC)
出版者:	WILEY
ISSN:	1520-4081
摘要:	Bisphenol A (BPA) is an estrogen-like compound, and an environmental hormone, that is commonly used in daily life. Therefore, it may enter the human body through food or direct contact, causing BPA residues in blood and urine. Because most studies focused on the analysis of BPA in reproductive cells or tissues, regarding evidence the effect of BPA on human retinal pigment epithelium (ARPE-19) cells unavailable. Accordingly, the present study explored the cytotoxicity of BPA on ARPE-19 cells. After BPA treatment, the expression of Bcl-XL an antiapoptotic protein, in the mitochondria decreased, and the expression of Bax, a proapoptotic protein increased. Then the mitochondrial membrane potential was affected. BPA changed in mitochondrial membrane potential led to the release of cytochrome C, which activated caspase-9 to promote downstream caspase-3 leading to cytotoxicity. The nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase 1 (HO-1) pathway play a major role in age-related macular degeneration. Our results showed that expression of HO-1 and Nrf2 suppressed by BPA. Superoxide dismutase and catalase, which Nrf2 downstream antioxidants, were degraded by BPA. AMP-activated kinase (AMPK), which can regulate the phosphorylation of Nrf2, and the phosphorylation of AMPK expression was reduced by BPA. Finally, BPA-induced ROS generation and cytotoxicity were reduced by N-acetyl-l-cysteine. Taken together, these results suggest that BPA induced ARPE-19 cells via oxidative stress, which was associated with down regulated Nrf2/HO-1 pathway, and the mitochondria dependent apoptotic signaling pathway.
URI:	http://dx.doi.org/10.1002/tox.23384 https://www.webofscience.com/wos/woscc/full-record/WOS:000708488800001 https://ir.csmu.edu.tw:8080/handle/310902500/23372
關聯:	ENVIRONMENTAL TOXICOLOGY ,2022,v37,issue 1, P131-141
显示于类别:	[中山醫學大學研究成果] 期刊論文

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