Establishing the blood reference interval of pancreatic elastase-1: A prospective study

doi:10.1111/jgh.15685

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/23314

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/23314

Title:	Establishing the blood reference interval of pancreatic elastase-1: A prospective study
Authors:	Chi, SF;Lai, CY;Dong, WT;Wang, JM;Wang, YY;Yang, SS;Lin, JT;Lee, TY
Keywords:	acute pancreatitis;biomarker;immunoreactive;pancreatic cancer
Date:	2021
Issue Date:	2022-08-05T09:35:16Z (UTC)
Publisher:	WILEY
ISSN:	0815-9319
Abstract:	Background and Aim Pancreatic elastase-1 (PE-1) has been investigated in pancreatic disorders. However, the reference interval (RI) of PE-1 in blood remains unconfirmed. We aimed to establish the blood RI of PE-1 in an adult population. Methods In this prospective cross-sectional study, we enrolled 400 adults who had received the whole-body physical check-up program between May 1, 2019 and November 20, 2019. The serum and plasma PE-1 levels were measured by latex turbidimetric immunoassay in different storage conditions (fresh, refrigerated, and frozen). The 95% and 99% RI of PE-1 were calculated according to the Clinical & Laboratory Standards Institute guidelines. The correlations between PE-1 and other parameters were analyzed using multivariable regression models. Ultimately, 38 patients with acute pancreatitis were prospectively recruited as the validation cohort. Results The PE-1 levels in fresh serum were highly correlated with those in refrigerated (R-2 = 0.998) or frozen (R-2 = 0.942) samples; however, plasma should not be suggested in frozen conditions (plasma vs serum: R-2 = 0.185). In the RI study population (202 male & 198 female participants), the median age was 52.6 (25-75% interquartile range: 43.1-61.0). The 95% and 99% RIs of PE-1 were 30.0-221.0 and 22.0-359.0 ng/dL, respectively. Triglycerides (beta = 0.106, P = 0.033), lipase (beta = 0.154, P = 0.007), and CA19-9 (beta = 0.130, P = 0.008) were independent factors associated with PE-1. In the pancreatitis validation cohort, with a cut-off value of 359.0 ng/dL, the sensitivity and specificity were 100% and 99.8%, respectively. Conclusion The RI of PE-1 established in this study can be used for further applications. Serum is the suggested form for frozen sample storage.
URI:	http://dx.doi.org/10.1111/jgh.15685 https://www.webofscience.com/wos/woscc/full-record/WOS:000701313500001 https://ir.csmu.edu.tw:8080/handle/310902500/23314
Relation:	JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY ,2022,v37,issue 1, P117-123
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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