Metformin induces apoptosis and inhibits migration by activating the AMPK/p53 axis and suppressing PI3K/AKT signaling in human cervical cancer cells

doi:10.3892/mmr.2020.11725

中山醫學大學機構典藏 CSMUIR > 中山醫學大學研究成果 > 期刊論文 > Item 310902500/23284

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/23284

Title:	Metformin induces apoptosis and inhibits migration by activating the AMPK/p53 axis and suppressing PI3K/AKT signaling in human cervical cancer cells
Authors:	Chen, YH;Yang, SF;Yang, CK;Tsai, HD;Chen, TH;Chou, MC;Hsiao, YH
Keywords:	metformin;apoptosis;migration;AMPK-activated protein kinase;p53;PI3K;AKT;cervical cancer
Date:	2021
Issue Date:	2022-08-05T09:34:46Z (UTC)
Publisher:	SPANDIDOS PUBL LTD
ISSN:	1791-2997
Abstract:	Human cervical cancer is the fourth most common malignancy among women worldwide, and it is expected to result in 460,000 deaths per year by 2040. Moreover, patients with cervical cancer often display drug resistance and severe side effects; therefore, the development of effective novel chemotherapeutic agents is important. In the present study, the effects of metformin, a first-line therapeutic drug for type 2 diabetes mellitus, were evaluated in cervical cancer. Compared with the control group, metformin significantly inhibited cell viability and migration, and induced apoptosis and cell cycle arrest in human cervical cancer cell lines (CaSki and HeLa). Following metformin treatment, the protein expression levels of p-AMP-activated protein kinase (p-AMPK), which promotes cell death, and the tumor suppressor protein p-p53 were remarkably upregulated in CaSki and C33A cells compared with the control group. Furthermore, compared with the control group, metformin significantly suppressed the PI3K/AKT signaling pathway in CaSki, C33A and HeLa cells. Compound C (an AMPK inhibitor) significantly reversed the effects of metformin on CaSki, C33A and HeLa cell viability, and AMPK and p53 phosphorylation. The results of the present study suggested that metformin induced AMPK-mediated apoptosis, thus metformin may serve as a chemotherapeutic agent for human cervical cancer.
URI:	http://dx.doi.org/10.3892/mmr.2020.11725 https://www.webofscience.com/wos/woscc/full-record/WOS:000598683200001 https://ir.csmu.edu.tw:8080/handle/310902500/23284
Relation:	MOLECULAR MEDICINE REPORTS ,2021,v23,issue 1
Appears in Collections:	[中山醫學大學研究成果] 期刊論文

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