miR-200a inhibits proliferation rate in drug-induced gingival overgrowth through targeting ZEB2

doi:doi.org/10.1016/j.jfma.2020.04.031

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 口腔醫學研究所 > 期刊論文 > Item 310902500/22358

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.csmu.edu.tw:8080/ir/handle/310902500/22358

题名:	miR-200a inhibits proliferation rate in drug-induced gingival overgrowth through targeting ZEB2
作者:	Lin, Taichen;Yu, Cheng-Chia;Liao, Yi-Wen;Hsieh, Pei-Ling;Chu, Pei-Ming;Liu, Chia-Ming;Yu, Chuan-Hang;Su, Tzu-Rong
关键词:	Cyclosporine A;Gingival overgrowth;MicroRNA-200a;ZEB2.
日期:	2020-08
上传时间:	2022-05-30T03:29:39Z (UTC)
出版者:	Elsevier Inc.
摘要:	Background/purpose: Gingival overgrowth can occur as a result of poor oral hygiene or a side effect of taking certain medications, such as cyclosporine A (CsA). It has been shown that this immunosuppressant drug induces epithelial-to-mesenchymal transition (EMT) in the gingival epithelium but the associated molecular mechanism remains to be elucidated. Methods: We first assessed the relative expression of microRNA-200a (miR-200a) in response to the CsA treatment using qRT-PCR. Next, luciferase reporter assay was applied to examine whether miR-200a was able to regulate ZEB2 and Western blot was utilized to measure the expression of ZEB2 in normal human gingival fibroblasts (HGFs). To confirm the significance of miR-200a and ZEB2 in the CsA-induced gingival overgrowth, miR-200a inhibitor and shRNA mediated knockdown of ZEB2 were used and cell proliferation in HGFs was assessed by MTT assay. Results: The expression of miR-200a was dose-dependently downregulated following the CsA treatment. Luciferase reporter assay confirmed that ZEB2 was a direct downstream target regulated by miR-200a and ZEB2 was indeed increased after the administration of CsA. We demonstrated that knockdown of ZEB2 hampered the CsA-induced HGFs proliferation and the elevated cell proliferation due to inhibition of miR-200a was reversed by repression of ZEB2. Conclusion: Our results showed that insufficient miR-200a in HGFs caused by CsA administration may lead to gingival enlargement mediated by the upregulation of ZEB2. This finding supported that CsA-induced EMT contributed to the adverse effect of using CsA and miR-200a may serve as an upstream target to prevent the overgrowth of the gingiva.
URI:	https://ir.csmu.edu.tw:8080/handle/310902500/22358
關聯:	J Formos Med Assoc,119(8),1299-1305
显示于类别:	[口腔醫學研究所] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
1-s2.0-S0929664620301698.pdf		841Kb	Adobe PDF	93	检视/开启

检视Licence

在CSMUIR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....