Docosahexaenoic acid inhibits TNFα-induced ICAM-1 expression by activating PPARα and autophagy in human endothelial cells

doi:doi.org/10.1016/j.fct.2019.110811

中山醫學大學機構典藏 CSMUIR > 健康管理學院 > 營養學系暨碩士班 > 期刊論文 > Item 310902500/22339

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/22339

Title:	Docosahexaenoic acid inhibits TNFα-induced ICAM-1 expression by activating PPARα and autophagy in human endothelial cells
Authors:	Lin, Hung-Chih;Lii, Chong-Kuei;Lin, Ai-Hsuan;Li, Chien-Chun;Tsai, Chia-Han;Pan, Shiuan-Kai;Yang, Ya-Chen;Huang, Chin-Shiu;Reshi, Latif;Chen, Haw-Wen
Contributors:	中山醫學大學;營養系
Keywords:	Autophagy;Docosahexaenoic acid (DHA);Intercellular adhesion molecule 1 (ICAM-1);Peroxisome proliferator-activated receptor alpha (PPARα);Tumor necrosis factor-alpha (TNFα)
Date:	2019-12
Issue Date:	2022-05-17T03:12:06Z (UTC)
Publisher:	Elsevier Inc.
Abstract:	Inflammation plays a key role in the development of cardiovascular disease (CVD), and docosahexaenoic acid (DHA) is recognized to fight against CVD. PPARα belongs to the nuclear hormone receptor superfamily and can interfere with inflammatory processes. Autophagy can degrade inflammasome proteins and counteract inflammation. Overexpression of intercellular adhesion molecule (ICAM) 1 in endothelial cells contributes to monocyte migration into the vascular intima. Here we investigated the mechanisms by which DHA inhibits TNFα-induced ICAM-1 expression in EA. hy926 endothelial cells. DHA markedly activated PPARα and suppressed TNFα-induced ICAM-1 expression, ICAM-1 promoter activity, p65 nuclear translocation, NFκB and DNA binding activity, and THP-1 cell adhesion. PPARα knockdown abolished the ability of DHA to inhibit TNFα-induced ICAM-1 expression and THP-1 cell adhesion. The PPARα antagonist GW6471 reversed the inhibitory effect of DHA on TNFα-induced ICAM-1 expression, p65 nuclear translocation, NFκB and DNA binding activity, and THP-1 cell adhesion. DHA significantly activated autophagy as evidenced by the formation of autophagosomes and increased LC3II protein expression. By contrast, wortmannin, which inhibits autophagy, abrogated DHA-induced autophagy and the inhibition of TNFα-induced ICAM-1 protein expression by DHA. Our results suggest that DHA likely inhibits TNFα-induced ICAM-1 expression by activating PPARα and autophagy.
URI:	https://ir.csmu.edu.tw:8080/handle/310902500/22339
Relation:	Food Chem Toxicol,134,110811
Appears in Collections:	[營養學系暨碩士班] 期刊論文

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