a b s t r a c t
Objective. Currently, infections due to foreign-body reactions caused by bacteria or implantmaterials at the wound site are one of the major reasons for the failure of guided tis-sue regeneration (GTR) and guided bone regeneration (GBR) in clinical applications. Thepurpose of this study was to develop regeneration membranes with localized cobalt ionrelease to reduce infection and inflammation by polycaprolactone (PCL)/cobalt-substitutedhydroxyapatite (CoHA).Methods. The PCL composite membrane containing 20 wt% CoHA powders was preparedby solvent casting. The surface morphology, crystal structure, chemical composition andthermal properties of PCL composite membranes were characterized. The biocompatibil-ity, osteogenic differentiation and antibacterial properties of composite membrane werealso investigated. Then, in biodegradability was assessed by immersing phosphate buffersolution (PBS) for 6 months.Results. Physicochemical analyses revealed that CoHA is evenly mixed in the membranes andassistance reduce the crystallinity of PCL for getting more degradation amounts than PCLmembrane. Osteoblast cells culture on the membrane showed that the CoHA significantlyincreases cell proliferation and found the calcium deposition production increased over 90%compared with PCL after 7 days of culture. A good antibacterial effect was achieved by theaddition of CoHA powder. The results were confirmed by 2.4 times reduction of proliferationof Escherichia coli (E. coli) seeded on the composite membrane after 24 h. Immersing in PBSfor 6 months indicated that PCL–CoHA composite membrane has improved biodegradationand can continuously remove free radicals to reduce the inflammatory response.
