研究目的：光傷害導致的氧化壓力會造成視網膜異常。藏花素是番紅花和梔子中的主要活性成分之一，有很強的抗氧化能力。本研究的目的是驗證藏花素在小鼠光傷害模式下對視覺表現、視網膜組織與Müller cell的影響。 研究方法及資料：實驗小鼠分配到為空白組(n=8)與LED光暴露組，暴露組分為(1)光照組(n=9)， (2)低劑量藏花素組(n=7)口服0.25毫克/每公斤及(3) 高劑量藏花素組(n=7)口服5毫克/每公斤每天兩次。視力及對比敏感度之測量是以動眼反射為基礎。視網膜組織以顯微組織及免疫組織化學染色法分析。對視網膜組織與視覺表現的相關性作統計分析。 研究結果：光傷害小鼠出現視力與對比敏感度下降，錐細胞損傷損傷，Müller cell hypertrophic gliosis。藏花素治療組則改善高空間頻率的視力與對比敏感度下降，保護錐細胞，Müller cell與視網膜組織。 結論與建議：本實驗顯現藏花素提升視力與對比敏感度，對抗光傷害保護視網膜細胞組織的能力。 Purpose： Oxidative stress after photodamage have impact on retinal abnormal changes. Crocin has powerful antioxidant capacities in retina in some clinical studies. The study aim to investigate the effect of crocin on visual performance, integrity of retinal tissue and Müller cells in photodamaged mouse retinas in vivo. Methods and Materials： Female CD-1® (ICR) mice were assigned either to one blank control group (n=8) or one of three light-emitting diode (LED) light exposure groups: (1) vehicle treatment group (n=9), (2) low dose of crocin group (LED-crocin 0.25mgkg−1, n=7), and (3)high dose of crocin group (LED-crocin 5mgkg−1, n=7).Visual acuity (VA) and visual contrast sensitivity function(VCSF) were measured by optokinetic reflex (OKR) based method. Retinal tissue were analyzed by histological and immunohistochemical stain. The association between retinal Müller cell hypertrophic gliotic index and visual performance was analized. Results：Crocin improved VA and VCSF especially on high spatial frequency. Crocin preserve retinal tissue and inhibit the mislocalization of cone opsin against photodamage. Crocin inhibited photodamage induced retinal Müller cell hypertrophic gliosis in vivo and associated with visual performance preservation. Conclusion: This study proved the protective effect of crocin against photodamage of retina in mice
