過去研究發現假孕鼠與真孕鼠之子宮蛻膜?殖期與蛋白激酵素C異構體的蛋白質表現有關。現今的研究,我們利用RT-PCR方式測量蛋白激酵素C異構體與下游相關基因的mRNA表現,再以DNA定序分析確定RT-PCR物之專一性。結果發現蛋白激酵素C異構體α mRNA在子宮蛻膜形成過程中第二到第七天有明顯增加,而同時期的轉錄活化因子(c-jun和c-fos)亦有明顯增加。這些變化說明蛋白激酵素C異構體α在子宮蛻膜組織處於活化的狀態。另外,也發現在蛻膜形成過程中蛋白激酵素C異構體λ和蛋白激酵素C異構體δ mRNA表現量增加。蛋白激酵素C異構體λ mRNA表現增加與細胞增殖指標(cdc2和cyclin D1)有類似的結果,然而蛋白激酵素C異構體δ mRNA在子宮蛻膜後期表現增加,此點說明蛋白激酵素C異構體δ屬於負修飾作用的角色。綜合以上結果,證實蛋白激酵素C異構體參與子宮蛻膜瘤形成的過程。
Our previous studies have demonstrated that the protein levels of PKC isoforms were altered in the proliferation stages of decidualization at pseudopregnant and pregnant rats. In this study, we measured the mRNA expressions of the PKC isoforms and their down-stream related genes by RT-PCR. The product of RT-PCR was also checked by DNA sequence analysis. The results showed that the levels of PKC mRNA were significantly increased from day 2 to day 7 in the decidualization. The expressions of the transcription factors (c-jun and c-fos) were also significantly increased during the same times. These specified that the alterations in the PKCα nmay signify their activation in decidual tissues. In addition, the mRNA levels of the other PKC isoforms (PKCλ and PKCδ) were also significantly increased during the decidualization. The increase in the PKCλ. mRNA was paralleled with the increase in the mRNA levels of the proliferation markers (cdc2 and cyclin Dl), while the PKCλ. mRNA was increased in the later stage of decidualization, supporting the role of PKCδ in negative- regulation. Thus, These data confirmed that the expression of PKC isoforms may be involved in the development of the deciduoma.