黃花蜜菜藥酒萃取物對人類多型性神經膠母細胞瘤細胞株之影響與相關訊息機制研究

中山醫學大學機構典藏 CSMUIR > 醫學院 > 生化微生物免疫研究所 > 博碩士論文 > Item 310902500/18787

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18787

Title:	黃花蜜菜藥酒萃取物對人類多型性神經膠母細胞瘤細胞株之影響與相關訊息機制研究 The effect and mechanism of Wedelia chinensis extract on human glioblastoma multiforme cells
Authors:	葉沛蓉 Yeh, Pei-Jung
Contributors:	曾博修
Keywords:	黃花蜜菜;神經膠母細胞瘤;腦癌 Wedelia chinensis;glioblastoma;brain cancer
Date:	2017
Issue Date:	2018-03-09T03:59:10Z (UTC)
Abstract:	多型性神經膠母細胞瘤 (glioblastoma multiforme，GBM) 是最常見且最具侵襲性的腦瘤，又稱為膠質細胞瘤 (glioblastoma)、第四級星形細胞瘤 (grade IV astrocytoma)，治療方式為手術切除後再與放射治療和化學治療同時治療。膠質細胞瘤產生的位置難以用手術完全去除，並且放射治療無可避免對重要腦區造成任何損傷。因此，對於惡性膠質瘤的治療方式是重要的課題。黃花蜜菜 (Wedelia chinensis (Osbeck) Merr.) 是台灣傳統的菊科中草藥，已經被證實具有抗發炎性質、保護肝臟的效果，並具有神經藥理學活性。過去文獻指出，黃花蜜菜萃取物可以抑制前列腺癌及乳癌細胞生長。但目前對於黃花蜜菜萃取物抑制膠質瘤細胞生長的機制尚未明確，因此本篇研究利用黃花蜜菜藥酒萃取物進行人類神經膠母細胞瘤細胞株之影響與相關訊息機制研究。　　本篇研究以細胞存活率試驗證實黃花蜜菜萃取物確實會抑制人類神經膠母細胞瘤細胞株 (GBM8401和U-87MG) 生長，並且抑制GBM8401細胞移動能力。此外在GBM8401細胞處理黃花蜜菜萃取物發現Sub G1有增加的情形，同時也觀察到p21蛋白表現增加、cyclin D1蛋白表現減少；與內源性凋亡路徑的相關蛋白表現中，抗凋亡蛋白Bcl-2 蛋白表現減少，促凋亡蛋白Bax、Cytochrome C、Apaf-1、Caspase-9及Caspase-3蛋白表現增加。由這些實驗結果顯示黃花蜜菜萃取物可誘導GBM8401細胞細胞週期停滯和凋亡，並表明黃花蜜菜在未來可能有助於提供GBM治療藥物的替代選擇。 Glioblastoma multiforme (GBM), known as glioblastoma and grade IV astrocytoma, is the most common and most aggressive brain cancer with the worst prognosis and survival time. Standard treatment for GBM includes surgical resection, combination with radiotherapy and chemotherapy. However, the anatomic location of gliomas makes it very difficult to surgically remove the tumor and it is impossible to avoid any damage to vital brain regions with radiotherapy. Therefore, therapeutic strategies for malignant glioma are urgently required improved. Wedelia chinensis is known as a traditional Chinese herbal medicine has been shown to have anti-inflammatory properties, anti-hepatic toxicity, and neuropharmacological activity. Recent evidences have suggested that Wedelia chinensis extract inhibits the growth of prostate cancer and breast cancer cells. However, little is known about the effects of Wedelia chinensis extract on glioblastoma. We herein intend to investigate the effect of Wedelia chinensis extract on human glioblastoma cells. 　　In this study, significantly decreased survival ratios of GBM8401 and U-87MG cells were detected in the presence of Wedelia chinensis extract. Significantly decreased migration was detected in GBM8401 cells treated with Wedelia chinensis extract. Additionally, significantly increased sub-G1 population was observed in GBM8401 cells that were treated with Wedelia chinensis extract as well the significantly increased P21 and decreased cyclin D1 proteins, significantly decreased Bcl-2 (anti-apoptotic) proteins and increased Bax (pro-apoptotic), Cytochrome C, Apaf-1, Caspase-9, Caspase-3 were detected by Western blot analysis. Altogether, these results demonstrated that treatment of GBM8401 cells with Wedelia chinensis extract induced cell cycle arrest and apoptosis, suggesting Wedelia chinensis as a useful alternative choice in treatment of GBM.
URI:	https://ir.csmu.edu.tw:8080/ir/handle/310902500/18787
Appears in Collections:	[生化微生物免疫研究所] 博碩士論文

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