本研究以新生大鼠腦組織之分離培養法，經初代培養，續代培養，以及利用各種純化分離方法，而獲得的純化之大鼠腦部組織星形神經膠細胞這些細胞以光學顯微鏡，掃描及穿透式電子顯微鏡，免疫細胞化學法及免疫螢光染色法等一系列之實驗研究，由其結果得到下列幾點結論一、本研究之腦組織細胞之分離培養技術較傳統之分離培養技術在時間上可縮短許多，而細胞於長期培養時可獲良好之生長及增殖。二、利用免疫細胞化學法及免疫螢光染色法所獲一致之結果，給予吾人極大之信心來肯定此大鼠腦部細胞之細胞類型確實隸屬於星形神經膠細胞。三、吾人可長時間培養星形神經膠細胞而不會失去它的分化特徵，即仍然可保持形成和貯存神經膠細胞纖維狀酸性蛋白的能力。總之，此大鼠腦部星形神經膠細胞已能適應體外長時間培養，並保有分化的星形神經膠細胞之形態特徵，相信對於今後星形神經膠細胞的形態，構造，生理機能和生化特性，以及其他相關之深 入研究必有裨益。 Dissociated cell culture of brain tissue from neonatal rat was described. From the primary culture to the long-term cult- ure, these cells are well-chraterized by a series of studies including light microscopies, scanning and transmission elect- ric microscopies, also by immunocytochemistry and immunofluor- escence assays. These result strongly suggest that: 1.This te- chnique of preparing and maintaining these brain tissue cells in vitro was relative easy and provide good condition of grow- th and proliferation. 2.It correlates well between immunocyto- chemical and immunofluorescence reactions, these give us great confidence in characterization of these cells as astrocytes. 3 .It is possible to maintain the astrocytes cell for long-term cultures without losing their differentiated characteristics mainly for the ability to sythesize and preserve glial fibri- llary acidic protein. We conclude: After long-term cultivation ,these astrocyte can be classified as a well-differentiated astrocytic type of glial cells, and would be useful model for further studies of morphology, function, biochemistry and re- lative studies of astrocytes in culture.
