Cytotoxicity evaluation of perforation repair materials on human periodontal ligament cells in vitro

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18404

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18404

Title:	Cytotoxicity evaluation of perforation repair materials on human periodontal ligament cells in vitro
Authors:	Tai, K.-W.;Chang, Y.-C.
Date:	2000
Issue Date:	2017-08-14T08:51:24Z (UTC)
ISSN:	0099-2399
Abstract:	Perforation of a tooth structure resulting in communication of the pulp space with periodontium occasionally occurs during endodontic therapy. For the best prognosis, the perforation area must be sealed as soon as possible. Because these materials will be in direct contact with periodontal tissues, their cytotoxic potential must be evaluated before clinical use. The purpose of this study was to determine the cytocompatibility of three perforation repair materials (amalgam, resin, and glass ionomer). Cultured human periodontal ligament (PDL) cells were used to evaluate the cellular response resulting from these materials by cell viability and proliferation assays. Twenty-seven 5 x 4 mm cylinders of each material were fabricated for this study. All tested materials were cytotoxic to human PDL cells. Both types of material and time affected cell viability and proliferation. Resin exhibited the most cytotoxic effects followed by glass ionomer and amalgam during a 14-day incubation period. Amalgam and glass ionomer slightly inhibited cell viability and growth in the first 24 hr, compared with the control. Amalgam or glass ionomer may initially react more favorably to PDL cells than resin. The present model of cultured human PDL cells is simple, relatively cheap, and easily established and propagated under standardized conditions in any laboratory. Furthermore, this method allows long-term observation of human cellular reactions and thus might be a preliminary screening test for initial biocompatibility of dental materials. Copyright © 2000 by The American Association of Endodontists.
URI:	http://dx.doi.org/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034230758&partnerID=40&md5=596bcf0357a2812d07f3b5dd4feffd59 https://ir.csmu.edu.tw:8080/ir/handle/310902500/18404
Relation:	Journal of Endodontics 26 (7) ,395-397
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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