Dentin bonding agents induce c-fos and c-jun protooncogenes expression in human gingival fibroblasts

doi:10.1016/S0142-9612(02)00273-9

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18382

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18382

Title:	Dentin bonding agents induce c-fos and c-jun protooncogenes expression in human gingival fibroblasts
Authors:	Huang, F.-M.;Chou, M.-Y.;Chang, Y.-C.
Keywords:	c-fos;c-jun;Dentin bonding agents;Primary culture;Protooncogene
Date:	2003
Issue Date:	2017-08-14T08:49:54Z (UTC)
ISSN:	0142-9612
Abstract:	An important requirement for a dentin bonding agent is biologic compatibility; the bonding agent usually remains in close contact with living dental tissues over a long period of time. Information on the genotoxicity/mutagenicity and cacinogenicity potentials of dentin bonding agents is rare. It has been shown that c-fos and c-jun are induced rapidly by a variety of chemical and physical stimuli. Little is known about the induction of cellular signaling events and specific gene expression after cell exposure to dentin bonding agents. Therefore, we used primary human gingival fibroblasts to examine the effect of six dentin bonding agents on the expression of c-fos and c-jun protooncogenes to evaluate the genotoxicity/mutagenicity and cacinogenicity potential of the dentin bonding agents. The levels of mRNA were measured by the quantitative RT-PCR analysis. c-fos and c-jun mRNA expression in dentin bonding agents-treated cells revealed a rapid accumulation of the transcript, a significant signal first was detectable after 1h of exposure. Persistent induction of c-jun and c-fos protooncogenes by dentine bonding agents may distribute systemically to cause some unexpected adverse effects on human beings. It would be necessary to identify the severely toxic compounds and replace these substances by better biocompatible components. Otherwise, leaching of those genotoxicity/mutagenicity and cacinogenicity components must be minimized or prevented. © 2002 Elsevier Science Ltd. All rights reserved.
URI:	http://dx.doi.org/10.1016/S0142-9612(02)00273-9 https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037210077&doi=10.1016%2fS0142-9612%2802%2900273-9&partnerID=40&md5=1b2c095c8bff4ae58cff4c709f2e64f0 https://ir.csmu.edu.tw:8080/ir/handle/310902500/18382
Relation:	Biomaterials 24 (1) ,157-163
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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