Examination of the signal transduction pathways leading to upregulation of tissue type plasminogen activator by Porphyromonas endodontalis in human pulp cells

doi:10.1111/j.1365-2591.2005.01026.x

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18357

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18357

Title:	Examination of the signal transduction pathways leading to upregulation of tissue type plasminogen activator by Porphyromonas endodontalis in human pulp cells
Authors:	Huang, F.-M.;Chen, Y.-J.;Chou, M.-Y.;Chang, Y.-C.
Keywords:	Casein zymography;Porphyromonas endodontalis;Pulp cells;Signal transduction pathway;Tissue type plasminogen activator
Date:	2005
Issue Date:	2017-08-14T08:48:08Z (UTC)
ISSN:	0143-2885
Abstract:	Aim: To investigate the tissue type plasminogen activator (t-PA) activity in human pulp cells stimulated with Porphyromonas endodontalis (P. endodontalis) in the absence or presence of p38 inhibitor SB203580, mitogen-activated protein kinase kinase (MEK) inhibitor U0126 and phosphatidylinositaol 3-kinase (PI3K) inhibitor LY294002. Methodology: The supernatants of P. endodontalis were used to evaluate t-PA activity in human pulp cells using casein zymography and enzyme-linked immunosorbent assay (ELISA). Furthermore, to search for possible signal transduction pathways, SB203580, U0126 and LY294002 were added to test how they modulated the t-PA activity. Results: The main casein secreted by human pulp cells migrated at 70 kDa and represented t-PA. Secretion of t-PA was found to be stimulated with P. endodontalis during 2-day cultured period (P < 0.05). From the results of casein zymography and ELISA, SB203580 and U0126 significantly reduced the P. endodontalis stimulated t-PA production respectively (P < 0.05). However, LY294002 lacked the ability to change the P. endodontalis stimulated t-PA production (P > 0.05). Conclusions: Porphyromonas endodontalis enhances t-PA production in human pulp cells, and the signal transduction pathways p38 and MEK are involved in the inhibition of t-PA. © 2005 International Endodontic Journal.
URI:	http://dx.doi.org/10.1111/j.1365-2591.2005.01026.x https://www.scopus.com/inward/record.uri?eid=2-s2.0-28944455243&doi=10.1111%2fj.1365-2591.2005.01026.x&partnerID=40&md5=659ef3c708a6665f2c59e4f12ab3f947 https://ir.csmu.edu.tw:8080/ir/handle/310902500/18357
Relation:	International Endodontic Journal 38 (12) ,860-865
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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