Effects of platelet-rich fibrin on human periodontal ligament fibroblasts and application for periodontal infrabony defects

doi:10.1111/j.1834-7819.2011.01362.x

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18319

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18319

Title:	Effects of platelet-rich fibrin on human periodontal ligament fibroblasts and application for periodontal infrabony defects
Authors:	Chang, Y.-C.;Zhao, J.-H.
Keywords:	alkaline phosphatase;osteoprotegerin;periodontal infrabony defects;periodontal ligament fibroblasts;Platelet-rich fibrin
Date:	2011
Issue Date:	2017-08-14T08:45:19Z (UTC)
ISSN:	0045-0421
Abstract:	Background: Platelet-rich fibrin (PRF) by Choukroun's technique is derived from an autogenous preparation of concentrated platelets. Little is known about the effects of PRF on periodontal ligament fibroblasts (PDLFs) and the application of PRF for periodontal regeneration. Methods: PDLFs were derived from healthy individuals undergoing extraction for orthodontic reasons. Blood collection was carried out from healthy volunteers. PRF was obtained from a table centrifuge centrifuged at 3000 rpm for 12 minutes. The effects of PRF on PDLFs were determined by measuring the expression of phosphorylated extracellular signal-regulated protein kinase (p-ERK), osteoprotegerin (OPG) and alkaline phosphatase (ALP) activity. Moreover, we retrospectively examined the feasibility and safety of reconstructing the periodontal infrabony defects with PRF in six patients. Results: PRF was found to increase ERK phosphorylation and OPG in PDLFs in a time-dependent manner (p < 0.05). ALP activity was also significantly upregulated by PRF (p < 0.05). Application of PRF in infrabony defects exhibited pocket reduction and clinical attachment gain after six months. Periapical radiography revealed radiographic defect filled in grafted teeth. Conclusions: The enhancement of p-ERK, OPG and ALP expression by PRF may provide benefits for periodontal regeneration. Clinical and radiologic analysis showed that the use of PRF is an effective modality for periodontal infrabony defects. © 2011 Australian Dental Association.
URI:	http://dx.doi.org/10.1111/j.1834-7819.2011.01362.x https://www.scopus.com/inward/record.uri?eid=2-s2.0-82455212418&doi=10.1111%2fj.1834-7819.2011.01362.x&partnerID=40&md5=8c4ef953d7965fc2d719fef0d0a61851 https://ir.csmu.edu.tw:8080/ir/handle/310902500/18319
Relation:	Australian Dental Journal 56 (4) ,365-371
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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