Root canal sealers cause cytotoxicity and oxidative damage in hepatocytes

doi:10.1002/1097-4636(20010305)54:3<390::AID-JBM110>3.0.CO;2-U

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18292

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18292

Title:	Root canal sealers cause cytotoxicity and oxidative damage in hepatocytes
Authors:	Hunag, T.-H.;Lii, C.-K.;Kao, C.-T.
Keywords:	Cytotoxicity;Glutathione;LDH leakage;Oxidative stress;Root canal sealer
Date:	2001
Issue Date:	2017-08-09T04:45:08Z (UTC)
Publisher:	John Wiley & Sons Inc, New York, NY, United States
ISSN:	219304
Abstract:	Elutable substances and degradation products from root canal sealers may gain access to periodontal tissue in many ways. The aim of this study was to investigate damage and oxidative stress caused by treatment with root canal sealers in primary cultured rat hepatocytes. Four root canal sealers were used: Canals, Sealapex, AH 26, and AH Plus. Toxicity was detected by assessing lactate dehydrogenase (LDH) leakage, and oxidative stress was determined by analysis of glutathione (GSH) levels. One-way analysis of variance was performed on the data. As the concentrations of sealer were increased, LDH leakage increased (p < 0.01). Increased GSH depletion accompanied LDH leakage increase. GSH levels were reduced to zero at a concentration of 0.05% for Canals and 0.10% for AH 26. These results confirmed the hypothesis that root canal sealers can cause cytotoxicity in hepatocytes. The Canals and AH 26 sealers can also cause oxidative stress in hepatocytes. © 2000 John Wiley & Sons, Inc.Elutable substances and degradation products from root canal sealers may gain access to periodontal tissue in many ways. The aim of this study was to investigate damage and oxidative stress caused by treatment with root canal sealers in primary cultured rat hepatocytes. Four root canal sealers were used: Canals, Sealapex, AH 26, and AH Plus. Toxicity was detected by assessing lactate dehydrogenase (LDH) leakage, and oxidative stress was determined by analysis of glutathione (GSH) levels. One-way analysis of variance was performed on the data. As the concentrations of sealer were increased, LDH leakage increased (p<0.01). Increased GSH depletion accompanied LDH leakage increase. GSH levels were reduced to zero at a concentration of 0.05% for Canals and 0.10% for AH 26. These results confirmed the hypothesis that root canal sealers can cause cytotoxicity in hepatocytes. The Canals and AH 26 sealers can also cause oxidative stress in hepatocytes.
URI:	http://dx.doi.org/10.1002/1097-4636(20010305)54:3<390::AID-JBM110>3.0.CO;2-U https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035809840&doi=10.1002%2f1097-4636%2820010305%2954%3a3%3c390%3a%3aAID-JBM110%3e3.0.CO%3b2-U&partnerID=40&md5=9dba754a9cfc0c249d05072a61acabfb https://ir.csmu.edu.tw:8080/ir/handle/310902500/18292
Relation:	Journal of Biomedical Materials Research 54(3) ,390-395
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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