Activation of focal adhesion kinase induces extracellular signal-regulated kinase-mediated osteogenesis in tensile force-subjected periodontal ligament fibroblasts but not in osteoblasts

doi:10.1007/s00774-013-0549-3

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18243

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18243

Title:	Activation of focal adhesion kinase induces extracellular signal-regulated kinase-mediated osteogenesis in tensile force-subjected periodontal ligament fibroblasts but not in osteoblasts
Authors:	Chen, Y.-J.;Shie, M.-Y.;Hung, C.-J.;Wu, B.-C.;Liu, S.-L.;Huang, T.-H.;Kao, C.-T.
Keywords:	Focal adhesion kinase (FAK);Osteoblast;Osteogenic;Periodontal ligament (PDL);Tensile force
Date:	2014
Issue Date:	2017-08-09T04:43:01Z (UTC)
Publisher:	Springer-Verlag Tokyo
ISSN:	9148779
Abstract:	The exact mechanism by which focal adhesion kinase (FAK) translates mechanical signals into osteogenesis differentiation in force-subjected cells has not been elucidated. The responses to different forces differ according to the origin of cells and the type of stress applied. Therefore, the recruitment of osteoclast and osteoblast progenitor cells, and the balanced activation of these cells around and within the periodontal ligament (PDL) are essential for alveolar bone remodeling. Cells within the PDL and MG63 cells were subjected to tensile forces of −100 kPa for different periods of time. At various times during the tensile force application, they were processed for the purpose of analyzing cell viability, cell cycle, and osteogenic protein. The effect of small interfering RNA transfection targeting FAK was also evaluated. Tensile force enhanced a rapid increase in the phosphorylation of FAK and up-regulated osteogenic protein expression in PDL cells, but not in MG63 cells. Transfecting PDL cells with FAK antisense oligonucleotide diminished alkaline phosphatase and osteocalcin secretion. These findings suggest that tensile force activates FAK pathways in PDL cells, which down-regulate immune cytokine and up-regulate osteogenic protein. © 2013, The Japanese Society for Bone and Mineral Research and Springer Japan.
URI:	http://dx.doi.org/10.1007/s00774-013-0549-3 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84911806106&doi=10.1007%2fs00774-013-0549-3&partnerID=40&md5=a9d05327e2f16c13bc8ab461c8e81e8e https://ir.csmu.edu.tw:8080/ir/handle/310902500/18243
Relation:	Journal of Bone and Mineral Metabolism 32(6) ,671-682
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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