Antiosteoclastogenesis activity of a CO2 laser antagonizing receptor activator for nuclear factor kappaB ligand-induced osteoclast differentiation of murine macrophages

doi:10.1088/1612-2011/12/3/035601

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18226

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18226

Title:	Antiosteoclastogenesis activity of a CO2 laser antagonizing receptor activator for nuclear factor kappaB ligand-induced osteoclast differentiation of murine macrophages
Authors:	Kuo, C.-L.;Kao, C.-T.;Fang, H.-Y.;Huang, T.-H.;Chen, Y.-W.;Shie, M.-Y.
Keywords:	CO2 laser;macrophage;osteoclastogenesis;receptor activator for nuclear factor kappa B;tartrate-resistant acid phosphatase
Date:	2015
Issue Date:	2017-08-09T04:42:16Z (UTC)
Publisher:	Institute of Physics Publishing
ISSN:	16122011
Abstract:	Macrophage cells are the important effector cells in the immune reaction which are indispensable for osteoclastogenesis; their heterogeneity and plasticity renders macrophages a primer target for immune system modulation. In recent years, there have been very few studies about the effects of macrophage cells on laser treatment-regulated osteoclastogenesis. In this study, RAW 264.7 macrophage cells were treated with RANKL to regulate osteoclastogenesis. We used a CO2 laser as a model biostimulation to investigate the role of osteoclastogenic. We also evaluated cell viability, cell death and cathepsin K expression. The CO2 laser inhibited a receptor activator of the NF-B ligand (RANKL)-induced formation of osteoclasts during the osteoclast differentiation process. It was also found that irradiation for two times reduced RANKL-enhanced TRAP activity in a dose-dependent manner. Furthermore, CO2 laser-treatment diminished the expression and secretion of cathepsin K elevated by RANKL and was concurrent with the inhibition of TRAF6 induction and NF-B activation. The current report demonstrates that CO2 laser abrogated RANKL-induced osteoclastogenesis by retarding osteoclast differentiation. The CO2 laser can modulate every cell through dose-dependent in vitro RANKL-mediated osteoclastogenesis, such as the proliferation and fusion of preosteoclasts and the maturation of osteoclasts. Therefore, the current results serve as an improved explanation of the cellular roles of macrophage cell populations in osteoclastogenesis as well as in alveolar bone remodeling by CO2 laser-treatment. © 2015 Astro Ltd.
URI:	http://dx.doi.org/10.1088/1612-2011/12/3/035601 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923684232&doi=10.1088%2f1612-2011%2f12%2f3%2f035601&partnerID=40&md5=15e3b71cc722f4f3800eab09efc14a5e https://ir.csmu.edu.tw:8080/ir/handle/310902500/18226
Relation:	Laser Physics Letters 12(3)
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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