Antibacterial and Odontogenesis Efficacy of Mineral Trioxide Aggregate Combined with CO<inf>2</inf> Laser Treatment

doi:10.1016/j.joen.2015.02.031

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18225

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18225

Title:	Antibacterial and Odontogenesis Efficacy of Mineral Trioxide Aggregate Combined with CO2 Laser Treatment
Authors:	Hsu, T.-T.;Yeh, C.-H.;Kao, C.-T.;Chen, Y.-W.;Huang, T.-H.;Yang, J.-J.;Shie, M.-Y.
Keywords:	Antibacterial;CO2 laser;human dental pulp cell;mineral trioxide aggregate;odontogenic
Date:	2015
Issue Date:	2017-08-09T04:42:13Z (UTC)
Publisher:	Elsevier Inc.
ISSN:	992399
Abstract:	Introduction Mineral trioxide aggregate (MTA) has been successfully used in clinical applications in endodontics. Studies show that the antibacterial effects of CO<inf>2</inf> laser irradiation are highly efficient when bacteria are embedded in biofilm because of a photothermal mechanism. The aim of this study was to confirm the effects of CO<inf>2</inf> laser irradiation on MTA with regard to both material characterization and cell viability. Methods MTA was irradiated with a dental CO<inf>2</inf> laser using directly mounted fiber optics in the wound healing mode with a spot area of 0.25 cm2 and then stored in an incubator at 100% relative humidity and 37°C for 1 day to set. The human dental pulp cells cultured on MTA were analyzed along with their proliferation and odontogenic differentiation behaviors. Results The results indicate that the setting time of MTA after irradiation by the CO<inf>2</inf> laser was significantly reduced to 118 minutes rather than the usual 143 minutes. The maximum diametral tensile strength and x-ray diffraction patterns were similar to those obtained without CO<inf>2</inf> laser irradiation. However, the CO<inf>2</inf> laser irradiation increased the amount of Ca and Si ions released from the MTA and regulated cell behavior. CO<inf>2</inf> laser-irradiated MTA promoted odontogenic differentiation of hDPCs, with the increased formation of mineralized nodules on the substrate's surface. It also up-regulated the protein expression of multiple markers of odontogenic and the expression of dentin sialophosphoprotein protein. Conclusions The current study provides new and important data about the effects of CO<inf>2</inf> laser irradiation on MTA with regard to the decreased setting time and increased ion release. Taking cell functions into account, the Si concentration released from MTA with laser irradiation may be lower than a critical value, and this information could lead to the development of new regenerative therapies for dentin and periodontal tissue. © 2015 American Association of Endodontists.
URI:	http://dx.doi.org/10.1016/j.joen.2015.02.031 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84937521111&doi=10.1016%2fj.joen.2015.02.031&partnerID=40&md5=611fe43b1e9c105b1a222ff0c1407ae9 https://ir.csmu.edu.tw:8080/ir/handle/310902500/18225
Relation:	Journal of Endodontics 41(7) ,1073-1080
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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