The synergistic effect on osteogenic differentiation of human mesenchymal stem cells by diode laser-treated stimulating human umbilical vein endothelial cells

doi:10.1088/1612-2011/13/2/025604

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18221

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18221

Title:	The synergistic effect on osteogenic differentiation of human mesenchymal stem cells by diode laser-treated stimulating human umbilical vein endothelial cells
Authors:	Kao, C.-T.;Hsu, T.-T.;Huang, T.-H.;Wu, Y.-T.;Chen, Y.-W.;Shie, M.-Y.
Keywords:	co-culture;diode laser;human mesenchymal stem cell;human umbilical vein endothelial cell;osteogenesis
Date:	2016
Issue Date:	2017-08-09T04:42:02Z (UTC)
Publisher:	Institute of Physics Publishing
ISSN:	16122011
Abstract:	Angiogenesis plays an important role in determining the biostimulation of bone regeneration, in either new bone or blood vessel formation. Human umbilical cord cells (HUVECs) are important effector cells in angiogenesis and are indispensable for osteogenesis and for their heterogeneity and plasticity. However, there are very few studies about the effects of HUVECs on diode laser-stimulated/regulated osteogenesis. In this study, we used diode laser as a model biostimulation to examine the role of HUVECs on laser-stimulated osteogenesis. Several bone formation-related proteins were also significantly up-regulated by the diode laser stimulation, indicating that HUVECs may participate in diode laser-stimulated osteogenesis. Interestingly, when human mesenchymal stem cells (hMSCs) cultured with HUVECs were diode laser-treated, the osteogenesis differentiation of the hMSCs was significantly promoted, indicating the important role of HUVECs in diode laser-enhanced osteogenesis. Adequately activated HUVECs are vital for the success of diode laser-stimulated hard-tissue regeneration. These findings provided valuable insights into the mechanism of diode laser-stimulated osteogenic differentiation, and a strategy to optimize the evaluation system for the in vitro osteogenesis capacity of laser treatment in periodontal repair. © 2016 Astro Ltd.
URI:	http://dx.doi.org/10.1088/1612-2011/13/2/025604 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84957673133&doi=10.1088%2f1612-2011%2f13%2f2%2f025604&partnerID=40&md5=5fc04d5f8dd1a50f0adff0b596de6af0 https://ir.csmu.edu.tw:8080/ir/handle/310902500/18221
Relation:	Laser Physics Letters 13(2) ,-
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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