Calcium silicate/chitosan-coated electrospun poly (lactic Acid) fibers for bone tissue engineering

doi:10.3390/ma10050501

中山醫學大學機構典藏 CSMUIR > 口腔醫學院 > 牙醫學系暨碩士班 > 期刊論文 > Item 310902500/18218

Please use this identifier to cite or link to this item: https://ir.csmu.edu.tw:8080/ir/handle/310902500/18218

Title:	Calcium silicate/chitosan-coated electrospun poly (lactic Acid) fibers for bone tissue engineering
Authors:	Su, C.-J.;Tu, M.-G.;Wei, L.-J.;Hsu, T.-T.;Kao, C.-T.;Chen, T.-H.;Huang, T.-H.
Keywords:	Calcium silicate;Chitosan;Osteogenesis;Poly (lactic acid);Tissue engineering
Date:	2017
Issue Date:	2017-08-09T04:41:53Z (UTC)
Publisher:	MDPI AG
ISSN:	19961944
Abstract:	Electrospinning technology allows fabrication of nano- or microfibrous fibers with inorganic and organic matrix and it is widely applied in bone tissue engineering as it allows precise control over the shapes and structures of the fibers. Natural bone has an ordered composition of organic fibers with dispersion of inorganic apatite among them. In this study, poly (lactic acid) (PLA) mats were fabricated with electrospinning and coated with chitosan (CH)/calcium silicate (CS) mixer. The microstructure, chemical component, and contact angle of CS/CH-PLA composites were analyzed by scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. In vitro, various CS/CH-coated PLA mats increased the formation of hydroxyapatite on the specimens' surface when soaked in cell cultured medium. During culture, several biological characteristics of the human mesenchymal stem cells (hMSCs) cultured on CS/CH-PLA groups were promoted as compared to those on pure PLA mat. Increased secretion levels of Collagen I and fibronectin were observed in calcium silicate-powder content. Furthermore, with comparison to PLA mats without CS/CH, CS10 and CS15 mats markedly enhanced the proliferation of hMSCs and their osteogenesis properties, which was characterized by osteogenic-related gene expression. These results clearly demonstrated that the biodegradable and electroactive CS/CH-PLA composite mats are an ideal and suitable candidate for bone tissue engineering. © 2017 by the authors.
URI:	http://dx.doi.org/10.3390/ma10050501 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019698522&doi=10.3390%2fma10050501&partnerID=40&md5=49dfdb7381017aa7d9090a8881962130 https://ir.csmu.edu.tw:8080/ir/handle/310902500/18218
Relation:	Materials 10(5) ,-
Appears in Collections:	[牙醫學系暨碩士班] 期刊論文

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